Fig. 6

Inhibition of fibrinogenolytic activity of SCF using zymogram protease assay. Arrows in red indicate zones of digestion of fibrinogen as substrate. SCF (4 μg/ml) was treated with protease inhibitors PMSF, EDTA, and DTT for 2 h and fibrinogenolytic activity of treated SCF was estimated post incubation. PC is positive control (untreated SCF), NC is negative control (20 mM KPB). Fibrinogenolytic activity was inhibited upon incubation of SCF with EDTA