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The Journal of Basic and Applied Zoology

Table 1 Effects of heat, protease, and specific serum complement inhibitors on the hemolysis of sheep red blood cells (SRBCs) by plasma from Crotalus viridis

From: Characterization of serum complement immune activity in the prairie rattlesnake (Crotalus viridis)

Treatment

SRBC hemolysis (% maximum)

Snake plasma

94.7 ± 1.2

Snake plasma (56 °C, 30 min)

1.1 ± 0.3*

Snake plasma + 20 mM ammonium hydroxide

91.9 ± 2.6

Snake plasma + 20 mM methylamine

92.1 ± 1.7

Snake plasma + 20 mM salicylaldoxime

6.5 ± 3.1*

Snake plasma + 5 mM EDTA

7.7 ± 2.4*

  1. Plasma from C. viridis was heat-treated, or treated with protease, EDTA, ammonium hydroxide, methylamine, or salicylaldoxime, and then incubated with 1% SRBCs for 30 min at room temperature. The samples were then centrifuged at 2000×g, and the optical densities were measured at 540 nm. The results represent the means ± standard deviations for four independent determinations and expressed as the % maximum as compared to a hemolysis positive control
  2. *Statistically less than SRBCs treated with plasma alone (p < 0.01)
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