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Table 2 Effects of divalent metal ions on the reconstitution of EDTA-inhibited sheep red blood cell (SRBC) hemolysis

From: Characterization of serum complement immune activity in the prairie rattlesnake (Crotalus viridis)

Treatment

SRBC hemolysis (% maximum)

Snake plasma

94.7 ± 1.2

Snake plasma + 5 mM EDTA

9.1 ± 1.6*

Snake plasma + 5 mM EDTA + 10 mM MgCl2

91.7 ± 3.6

Snake plasma + 5 mM EDTA + 10 mM CaCl2

93.3 ± 1.4

Snake plasma + 5 mM EDTA + 10 mM BaCl2

3.7 ± 0.8*

Snake plasma + 5 mM EDTA + 10 mM CuCl2

6.9 ± 1.4*

Snake plasma + 5 mM EDTA + 10 mM FeCl2

8.7 ± 1.9*

  1. Plasma from Crotalus viridis was treated EDTA, or EDTA plus and excess of Ca2+, Mg2+, Cu2+, Ba2+, or Fe2+, and then incubated with 1% SRBCs for 30 min at room temperature. The samples were then centrifuged at 2000×g, and the optical densities were measured at 540 nm and expressed as the % maximum as compared to a hemolysis positive control. The results represent the means ± standard deviations for four independent determinations
  2. *Statistically less than SRBCs treated with plasma alone (p < 0.01)
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