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Table 4 Rate of DNA damage in liver tissues of MMC-treated mice supplemented with different doses (50 and 100 mg/kg body wt) of Nitraria retusa leaf extracts at different time intervals (3 days, 1 week, and 1 month) using comet assay

From: Antioxidant capacity of Nitraria retusa leaf extracts against mitomycin C-induced genetic toxicity in male mice

Treatment

No. of cells

Class¥ of comet

DNA damaged cells (mean ± SEM)

Analyzed*

Total comets

0

1

2

3

Control

500

31

469

24

7

0

6.2 ± 0.26e

MMC

500

137

363

29

47

61

27.4 ± 0.22a

MMC + NR50 3d

500

113

387

31

38

44

22.6 ± 0.35b

MMC + NR100 3 days

500

104

396

28

35

41

20.8 ± 0.42bc

MMC + NR50 1w

500

91

409

23

32

36

18.2 ± 0.28c

MMC + NR100 1 week

500

74

426

24

29

21

14.8 ± 0.45cd

MMC + NR50 1 month

500

51

449

22

17

12

10.2 ± 0.33d

MMC + NR100 1 month

500

42

458

19

15

8

8.4 ± 0.25de

  1. ¥Class 0 = no tail; 1 = tail length < diameter of nucleus; 2 = tail length between 1× and 2× the diameter of nucleus; and 3 = tail length > 2× the diameter of nucleus. *No of cells analyzed were 100 per an animal
  2. a, b, c, d, e Different small superscript letters are differing significantly.