Fig. 3

Alignment of NEP sequences. NS2 protein (1–121) with reference strain A/ equine/Miami/1963 (the green line refers to the NES (nuclear export signal). NES, 11–23 aa; CRM1-binding domain, 1–58 aa; M1-binding domain, 59–116 aa (37). Accessions numbers: A/equine/Miami/1/1963, ABY81498; A/equine/Nador/1/1997, AFN69205; A/equine/Essaouira/2/2004, AFJ69908; A/equine/Essaouira/3/2004, AFJ69912; A/equine/Uruguay/1/1963, ACD85424; A/equine/Sao Paulo/1/1969, ACD85391; A/equine/Tokyo/2/1971, AEM60153; A/equine/Algiers/1/1972, ACF22132; A/equine/Kentucky/1/1978, ABY81443; A/equine/Fontainebleau/1/1979, ACD85402; A/equine/New Market/1/1979, AEM60119; A/equine/Georgia/1/1981, ABY81531; A/equine/Cordoba/18/1985, ACD85281; A/equine/Johannesburg/1/1986, ACF22143; A/eq/Kentucky/1/88, AAC31253; A/eq/LaPlata/1/88, AAC31255; A/equine/Berlin/1/1989, ACD85413; A/equine/Yvelines/2136/1989, AAC31257; A/equine/Rome/5/1991, AAC31263; A/equine/Kentucky/1/1992, ACA24640; A/equine/Italy/1199/1992, ACD85314; A/equine/Avesta/1/1993, BAI67723;A/equine/Newmarket/1/1993, ACI48793;A/equine/Kentucky/1/1998, ACH95656; A/equine/Ohio/1/2003, ABA42432; A/equine/Kentucky/9/2004, ACH95648;A/equine/Cheshire/1/2006, ACH95606; A/equine/Florida/2/2006, ACH95626; A/equine/California/1/2007, ACH95622; A/equine/Richmond/1/2007, ACH95618; A/equine/Athens/04/2007, ADT80593; A/equine/Kanazawa/1/2007, BAF95466; A/equine/Liaoning/9/2008, ACE81911; A/equine/Mysore/12/2008, ACU82372; A/equine/Gironde/1/2014, APQ31497