Effect of dietary Citrus sinensis peel extract on growth performance, digestive enzyme activity, muscle biochemical composition, and metabolic enzyme status of the freshwater fish, Catla catla

The Journal of Basic and Applied Zoology

Table 4 Activities of digestive and the metabolic enzymes (U/mg protein) in Catla catla fed with different concentration of Citrus sinensis-supplemented diets

Enzymes		Citrus sinensis concentration g kg^− 1
Enzymes			Control	2.00 (diet 1)	6.00 (diet 2)	10 (diet 3)
Digestive enzymes (U/mg protein)	Protease	Initial	0.71 ± 0.22	0.71 ± 0.22	0.71 ± 0.22	0.71 ± 0.22
	Protease	Final	1.11 ± 0.36^c	1.69 ± 0.19^bc	3.21 ± 0.65^a	2.25 ± 0.48^b
	Amylase	Initial	0.30 ± 0.03	0.30 ± 0.03	0.30 ± 0.03	0.30 ± 0.03
	Amylase	Final	0.58 ± 0.11^c	1.15 ± 0.40^bc	2.23 ± 0.55^a	1.56 ± 0.27^ab
	Lipase	Initial	0.28 ± 0.11	0.28 ± 0.11	0.28 ± 0.11	0.28 ± 0.11
	Lipase	Final	0.37 ± 0.06^b	0.62 ± 0.10^ab	1.15 ± 0.49^a	0.76 ± 0.26^ab
Metabolic enzymes (U/L)	GOT	Initial	9.05 ± 0.04	9.05 ± 0.04	9.05 ± 0.04	9.05 ± 0.04
	GOT	Final	15.03 ± 0.89^a	16.12 ± 2.75^a	17.79 ± 2.64^a	16.39 ± 1.06^a
	GPT	Initial	6.64 ± 2.11	6.64 ± 2.11	6.64 ± 2.11	6.64 ± 2.11
	GPT	Final	10.03 ± 0.94^a	12.98 ± 1.18^a	13.80 ± 3.32^a	13.75 ± 2.41^a

Mean values within the same row sharing the same superscript are not significantly different (P ˃ 0.05), Citrus sinensis-free diet; n = 3 (three samples from each treatment), mean ± SD