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Fig. 5 | The Journal of Basic and Applied Zoology

Fig. 5

From: AiP1, a protein from the coelomic fluid of sea star Astropecten indicus promotes wound healing and fibrinogenolysis in vitro

Fig. 5

Inhibition of fibrinogenolytic activity of AiP1 by EDTA: A Metalloproteinase nature of AiP1 was confirmed by estimating fibrinogenolytic activity of AiP1 post treatment with different inhibitors followed by incubating with fibrinogen to assess presence or loss of fibrinogenolytic activity post treatment. Lane 1: AiP1 treated with EDTA for 60 min; lane 2: AiP1 treated with EDTA for 30 min; lane 3: AiP1 treated with PMSF for 60 min; lane 4: untreated AiP1; lane 5: fibrinogen control. B Intensity of fibrinogen bands plotted against the AiP1 treated with various protease inhibitors. Digestion of Aα band of fibrinogen was maximum in untreated AiP1, AiP1 treated with PMSF, and AiP1 treated with EDTA for 30 min. Digestion of Bβ chain of fibrinogen was at maximum in untreated AiP1 followed by AiP1 treated with PMSF. *p < 0.05; **p < 0.01

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