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Table 3 Biochemical responses of E. eugeniae exposed to benzene and toluene contaminated soil

From: Histopathological and biochemical alterations in Eudrilus eugeniae (Kinberg 1867) as biomarkers of exposure to monocyclic aromatic hydrocarbons in oil impacted site

Biochemical measurements

Duration of exposure (days)

Benzene concentration

Toluene concentration

  

High (1/10th)

Low (1/100th)

High (1/10th)

Low (1/100th)

GSH

0

28.64 ± 1.47

28.64 ± 1.47

28.64 ± 1.47

28.64 ± 1.47

2

12.27 ± 1.22*

20.29 ± 1.30

10.73 ± 0.84*

13.81 ± 0.91*

7

12.51 ± 4.03*

16.70 ± 1.23

31.84 ± 1.1*

32.77 ± 1.08*

15

33.58 ± 2.89*

62.32 ± 32.13*

31.47 ± 1.04*

32.98 ± 1.35*

21

27.04 ± 1.18

29.36 ± 1.04

13.08 ± 1.31*

19.11 ± 1.04

28

34.52 ± 1.48*

37.02 ± 0.77

16.22 ± 1.25*

16.97 ± 1.15

SOD

0

235.20 ± 30.41

235.20 ± 30.41

235.20 ± 30.41

235.20 ± 30.41

2

105.46 ± 8.65*

195.75 ± 7.87*

81.94 ± 27.7*

69.88 ± 9.16*

7

94.84 ± 5.36*

45.52 ± 11.06*

50.07 ± 12.41*

68.28 ± 8.48*

15

94.84 ± 5.36*

45.52 ± 11.06*

182.54 ± 2.9*

64.64 ± 5.11*

21

115.70 ± 6.00*

124.73 ± 9.47*

64.11 ± 4.37*

72.08 ± 6*

28

97.11 ± 9.52*

96.05 ± 7.41*

39.23 ± 11.92

170.71 ± 24.88

CAT

0

693.00 ± 78.85

693.00 ± 78.85

693 ± 42.15

693 ± 42.15

2

449.75 ± 84.29*

585.90 ± 94.28*

470.05 ± 64.89*

489.3 ± 86.27

7

288.75 ± 19.57*

93.45 ± 7.38*

196 ± 38.12*

248.5 ± 64.77*

15

288.75 ± 19.57*

93.45 ± 7.38*

175.35 ± 16.94*

214.55 ± 24.52*

21

399.00 ± 33.66*

506.10 ± 28.93

156.8 ± 12.53*

225.4 ± 29.58*

28

354.90 ± 30.75*

430.85 ± 39.02

485.8 ± 62.04*

429.8 ± 85.49

MDA

0

2.46 ± 0.12

2.46 ± 0.12

2.46 ± 0.12

2.46 ± 0.12

2

7.81 ± 0.11*

4.74 ± 0.28

6.8 ± 0.72*

4.05 ± 0.52*

7

3.23 ± 0.06*

1.78 ± 0.07*

2.95 ± 0.13

2.32 ± 0.16

15

3.23 ± 0.06*

1.78 ± 0.07*

3.03 ± 0.16

2.32 ± 0.15

21

0.42 ± 0.10*

0.23 ± 0.04*

0.52 ± 0.12*

1.37 ± 0.15*

28

2.75 ± 0.15*

1.74 ± 0.11*

1.62 ± 0.82

1.78 ± 0.13

GST

0

395.77 ± 20.30

395.77 ± 20.30

395.77 ± 20.30

395.77 ± 20.30

2

395.77 ± 20.30

418.27 ± 6.32*

274.96 ± 16.6*

246.21 ± 15.55

7

280.37 ± 17.95*

169.56 ± 16.83*

234.55 ± 13.06*

190.8 ± 12.55*

15

363.69 ± 14.17

667.39 ± 29.70*

434.93 ± 14.31*

453.68 ± 19.4*

21

373.90 ± 16.20*

405.77 ± 10.66*

181.22 ± 17.34*

264.12 ± 14.31

28

477.01 ± 20.46

511.58 ± 14.40*

224.13 ± 18.8*

234.55 ± 15.84

  1. CAT Catalase, MDA Malondialdehyde, GSH Total glutathione, GST Glutathione-S-transferase, SOD Superoxide dismutase; statistical significance, treated versus control group:*(p < 0.05). Results are expressed as means ± standard deviations (n = 5)