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Table 1 Purification process of fat body lipase from G. mellonella larvae

From: Purification and characterization of fat body lipase from the greater wax moth, Galleria mellonella (Lepidoptera: Pyralidae)

Purification step

Proteina (mg/ml)

Total activityb (U)

Specific activityb (U/mg)

Purification fold

(%)

Crude extract

1.68 ± 0.0724 (A)

0.026767 ± 0.000681 (A)

0.015933 ± 0.000288 (B)

1

100

(NH4)2SO4 (0–40%)

0.903 ± 0.00608 (B)

0.024 ± 0.0 (B)

0.0266 ± 0.000177 (B)

1.67

89.7

(NH4)2SO4 (40–80%)

0.16633 ± 0.00551 (C)

0.014033 ± 0.000153 (C)

0.0844 ± 0.00216 (B)

5.3

52.43

Sephadex G-100

0.008633 ± 0.000551 (D)

0.0136 ± 0.0001 (C)

1.5754 ± 0.1042 (A)

98.9

50.81

  1. aProtein content was measured by the Bradford method (1976)
  2. bOne unit of activity corresponds to 1 μmol of pNP released per min using 50 mM pNPB as the substrate at 37 °C
  3. Data are showed as (mean ± SE), n = 3 replicates
  4. Different letters indicate significant differences (p < 0.05)