Purification step | Proteina (mg/ml) | Total activityb (U) | Specific activityb (U/mg) | Purification fold | (%) |
---|---|---|---|---|---|
Crude extract | 1.68 ± 0.0724 (A) | 0.026767 ± 0.000681 (A) | 0.015933 ± 0.000288 (B) | 1 | 100 |
(NH4)2SO4 (0–40%) | 0.903 ± 0.00608 (B) | 0.024 ± 0.0 (B) | 0.0266 ± 0.000177 (B) | 1.67 | 89.7 |
(NH4)2SO4 (40–80%) | 0.16633 ± 0.00551 (C) | 0.014033 ± 0.000153 (C) | 0.0844 ± 0.00216 (B) | 5.3 | 52.43 |
Sephadex G-100 | 0.008633 ± 0.000551 (D) | 0.0136 ± 0.0001 (C) | 1.5754 ± 0.1042 (A) | 98.9 | 50.81 |