Fig. 3
From: Successively expressed cuticular protein genes at the prepupal stage in wing discs of Bombyx mori
Induction of transcripts of ERTFs by different concentration of 20E. V4 wing discs were incubated for the indicated time in a medium containing indicated concentration of 20E. RNA was extracted from wing discs and reverse-transcribed to cDNA for use in qRT-PCR. Values were first normalized to rpl and then plotted relative to the 0 h value for each panel. Each datum was calculated from three independent experiments. The result is expressed as the mean ± S. E. M. Asterisks indicate p < 0.05 significance by the Student’s t test. Level of mRNA of the ecdysone treatment