Table 5 Biochemical characterizations of unused leech Gram-positive isolates
Sr.no. | Isolates → | UTL02 | UTL03 | UTL04 | UTL06 | UTL07 | UTL08 | UTL11 | UTL12 | UTL14 |
|---|---|---|---|---|---|---|---|---|---|---|
Name of test ↓ | ||||||||||
1 | Malanate production | -Ve | +ve | +ve | -Ve | -Ve | -Ve | -Ve | -Ve | +ve |
2 | V.P. test | -Ve | -Ve | -Ve | -Ve | -Ve | -Ve | -Ve | +ve | -Ve |
3 | Citrate utilization | -Ve | +ve | +ve | +ve | +ve | -Ve | -Ve | +ve | +ve |
4 | ONPG test | -Ve | -Ve | -Ve | -Ve | -Ve | -Ve | -Ve | -Ve | -Ve |
5 | Nitrate reduction | -Ve | -Ve | +Ve | -Ve | -Ve | -Ve | -Ve | +ve | +ve |
6 | Catalase test | -Ve | +ve | +ve | -Ve | +ve | -Ve | -Ve | +ve | +ve |
7 | Arginine test | -Ve | +ve | +ve | +ve | +ve | -Ve | -Ve | -Ve | +ve |
8 | Sucrose utilization | -Ve | -Ve | -Ve | +ve | -Ve | -Ve | -Ve | +ve | -Ve |
9 | Mannitol utilization | -Ve | -Ve | -Ve | -Ve | -Ve | -Ve | -Ve | -Ve | -Ve |
10 | Glucose utilization | +ve | +ve | -Ve | +ve | -Ve | -Ve | -Ve | +ve | +ve |
11 | Arabenose utilization | -Ve | +ve | +ve | -Ve | -Ve | +ve | -Ve | -Ve | +ve |
12 | Trehalose utilization | +ve | -Ve | -Ve | +ve | -Ve | -Ve | -Ve | +ve | -Ve |
13 | MR test | -Ve | +ve | -Ve | -Ve | +ve | +ve | -Ve | -Ve | +ve |
14 | Oxidase production | +ve | +ve | +ve | +ve | -Ve | -Ve | +ve | +ve | +ve |
15 | H2S test | -Ve | -Ve | -Ve | -Ve | -Ve | +ve | -Ve | -Ve | -Ve |
16 | Gelatin hydrolysis | -Ve | +ve | -Ve | -Ve | +ve | +ve | +ve | +ve | +ve |
17 | Starch hydrolysis | +ve | +ve | +Ve | +ve | +ve | +ve | +ve | -Ve | +ve |
18 | Casein hydrolysis | -Ve | -Ve | +ve | -Ve | +ve | +ve | -Ve | -Ve | -Ve |
Probable bacteria | Micrococcus lylae | Micrococcus luteus | Bacillus cereus | Streptococcus group F | Bacillus megaterium | Bacillus coagulans | Micrococcus lylae | Bacillus cereus | Bacillus megaterium | |