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The Journal of Basic and Applied Zoology
Fig. 5 | The Journal of Basic and Applied Zoology

Fig. 5

From: Caspase-8 in Labeo rohita is evolutionary conserved and is activated in Aeromonas hydrophila and Edwardsiella tarda infection and rhabdovirus vaccination

Fig. 5

Tissue-specific caspase-8 gene expression in Labeo rohita. To investigate the tissue-specific expression of caspase-8 gene, total RNA was extracted from the gill, liver, kidney, spleen, and blood of rohu fingerlings and cDNA was prepared. Employing quantitative real-time PCR (qRT-PCR) assay, the cDNA of each tissue sample was analyzed for the expression of caspase-8 and β-actin (housekeeping gene) gene expression. Expression of caspase-8 gene in each tissue was represented as a ratio relative to β-actin levels in the same samples. The spleen was chosen as the calibrator (1), and the relative expression of caspase-8 gene in other tissues was represented as fold changes from the calibrator. The results obtained from three fish were expressed as mean ± standard error (bars in the graph).

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