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The Journal of Basic and Applied Zoology
Fig. 7 | The Journal of Basic and Applied Zoology

Fig. 7

From: Caspase-8 in Labeo rohita is evolutionary conserved and is activated in Aeromonas hydrophila and Edwardsiella tarda infection and rhabdovirus vaccination

Fig. 7

Modulation of caspase-8 gene in Labeo rohita in response to E. tarda infection. Labeo rohita fingerlings (n = 3) were intra-peritoneally (i.p.) infected with E. tarda (3 × 106 CFU/fish), for 24, 48, and 72 h. Then, the gill, kidney, liver, spleen, and blood were collected from the un-infected control and E. tarda infected fish and total RNA was extracted. Following cDNA synthesis, caspase-8 and β-actin gene expression in each sample was analyzed by the qRT-PCR assay. Caspase-8 gene expression in each tissue has been normalized to the β-actin gene (housekeeping), and relative expression of caspase-8 gene (mean ± standard errors (bars)) has been represented as fold changes compared to the un-treated control fish (n = 3). a Gill. b Liver. c Kidney. d Spleen. e Blood

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