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The Journal of Basic and Applied Zoology
Fig. 9 | The Journal of Basic and Applied Zoology

Fig. 9

From: Caspase-8 in Labeo rohita is evolutionary conserved and is activated in Aeromonas hydrophila and Edwardsiella tarda infection and rhabdovirus vaccination

Fig. 9

Caspase-8 gene expression is induced in LRG cell line during cell death by A. hydrophila infection. Labeo rohita gill (LRG) cell line was cultured for 48 h, and then, it was either mock infected or A. hydrophila infected (at 1 MOI) for 2 h to 2.30 h. Then, control and treated cells were observed under microscope (× 10) to visualize the morphological changes. a Control, b at 2 h, and c at 2.30 h of A. hydrophila infection. d Total RNA was extracted from mock infected and A. hydrophila infected cells, and qRT- PCR was employed to investigate the expression of caspase-8 and β-actin gene. The caspase-8 gene expression has been normalized with the housekeeping gene β-actin and expressed as fold changes as compared to the control. The result has been expressed as mean ± standard errors (bars)

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