Fig. 7

Transmission electron microscope of brain section in ASP-LD and ASP-HD groups. a ASP-LD group showing junctions between capillary wall and neuropil (red arrow) with tight wall without any disintegration or edema. Pyknotic Purkinje cells appeared with irregular boundaries and abundant crystallizes mitochondria (CM) as well as fragmented rough endoplasmic reticulum (RER). Disintegration junction capillary wall between the capillary wall and astrocytic perivascular edema (PE) with the appearance of more vacuoles and vesiculated rough endoplasmic reticulum (VR). b TEM section of group treated with ASP-HD showing Purkinje cell with karyolysed nuclei and apoptotic appearance with irregular boundaries and convolution of the nuclear envelop with disintegration of unclear chromatin (***) having cytoplasm with very reduced mitochondria (*) and some crystalizes (CM) and modified cytological structures, the capillary junction between the capillary wall and astrocytic perivascular edema (PE) and thickening if the junction wall (black arrow) with very abnormal convolution appearance and congested blood vessels (CB) with moderate hemorrhage and appearance of more vacuoles