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Fig. 5 | The Journal of Basic and Applied Zoology

Fig. 5

From: Dandelion prevents liver fibrosis, inflammatory response, and oxidative stress in rats

Fig. 5

The expression of α-SMA collagens I and III positive cells of liver tissues of rats was determined by immunohistochemical satin (a and b) and by mRNA expression in the liver by quantitative RT-PCR (c). Representative histological pictures (a) and the semi-quantitative analysis (b) showing the expression of α-SMA and collagens I and III positive cells in different experimental groups. All pictures are × 100 magnifications. In control section, immunohistochemical staining is only revealed in vascular structures. In CCL4 sections, clear staining for α-SMA and collagens I and III is manifested along with the fibrous septa. The immunohistochemical expression of cells in each section was calculated by counting numbers of brown staining, α-SMA positive cells, in five fields per section at × 400 magnification. b Columns represent the means ± SEM for 6 animals per group. aP < 0.05 vs. control group; bP < 0.05 vs. CCL4 group. Analysis of α-SMA and types 1 and 3 collagen mRNA expression in the liver by quantitative RT-PCR (n = 3 per group). Data were normalized to GAPDH mRNA. Data are presented as means ± SEM. aP < 0.05 vs. control group; bP < 0.05 vs. CCL4 group; cP < 0.05 vs. dandelion group

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