Table 1 The gene-specific primers used for RT-PCR
From: Dandelion prevents liver fibrosis, inflammatory response, and oxidative stress in rats
Gene name | Primer sequence 5′-3′ | Gene bank accession number |
|---|---|---|
TNF-α | F: AACTCGAGTGACAAGCCCGTAG R: GTACCACCAGTTGGTTGTCTTTGA | |
NF-kB | F: CATTGAGGTGTATTTCACGG R: GAACACAATGGCCACTTGCC | NM_199267.2 |
IL-1β | F: GCTGTGGCAGCTACCTATGTCTTG R: AGGTCGTCATCATCCCACGAG | NM_031512.2 |
COX-2 | F: ACTTGCTCACTTTGTTTCATTC R: TTTGATTAGTACTGTAGGGTTAATG | S67722.1 |
TGF-β | F: TGCGCCTGCAGAGATTCAAG R: AGGTAACGCCAGGAATTGTTGCTA | NM_021578.2 |
α-SMA | F: ACCAACTGGGACGACATGGAG R: CGTGAGGATCTTCATGAGGTAGTC | NM_031004.2 |
Colla1 | F: GAACTTGGGGCAAGACAGTCA R: GTCACGTTCAGTTGGTCAA | NM_053304.1 |
Colla3 | F: TTGATGTGCAGCTGGCATTC R: GCCACTGGCCTGATCCATAT | NM_009930 |
GAPDH | F: CCCCTTCATTGACCTCAACTACATGG R: GCCTGCTTCACCACCTTCTTGATGTC | NM_017008.4 |