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Fig. 3 | The Journal of Basic and Applied Zoology

Fig. 3

From: The protective role of olive oil against gibberellic acid-induced embryotoxicity at prenatal stages of mice

Fig. 3

Photomicrographs of H&E-, PAS-, and TNFR2 immuno-stained sections in the ovary. Normal histological structure was observed in control ovary (a, c). GA3-treated group showed congestion of blood vessel (red arrow, b), degeneration of the follicles (black arrow, b), and vacuolation (black arrow, d). GA3-treated group showed alteration in the carbohydrates content (PAS) (arrow, f) as compared to the normal localization in the zona pellucida of control ovary (arrow, e). TNFR2 expression was detected in the zona pellucida of control ovary (arrow, g), whereas GA3-treated ovary showed undistinguished TNFR2 expression in zona pellucida (black arrow, h) with increase the TNFR2 expression in zona granulosa cells (red arrow, h). O, oocyte; F, follicle; N, nucleus; ZP, zona pellucida; ZG, zona granulose; TI, theca interna; TE, theca externa. Scale bar = 50 μm (a, b) and 10 μm (c–h)

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