Skip to main content
The Journal of Basic and Applied Zoology
Fig. 6 | The Journal of Basic and Applied Zoology

Fig. 6

From: Derivation, characterization, and in vitro cell regeneration of canine white adipose tissue-derived mesenchymal stem cells obtained from a mesenteric region

Fig. 6

Replicative senescence of canine MSCs. a Cells grown in DMEM-HG+ 10% FBS reached senescence at passage 9. b Same phenomenon was observed at passage 12 when cells were grown in DMEM-LG+ 20% FBS. Senescence cells were characterized by enlarged or flattened morphology and reduced proliferation capacity. Similarly, c d cell senescence in both culture medium as detected by senescence associated β-galactosidase activity. e Percentage of senescence cells at 9th and 12th passage. The data represent means ± SD. *p < 0.05 was considered statistically significant

Back to article page