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The Journal of Basic and Applied Zoology
Fig. 7 | The Journal of Basic and Applied Zoology

Fig. 7

From: Derivation, characterization, and in vitro cell regeneration of canine white adipose tissue-derived mesenchymal stem cells obtained from a mesenteric region

Fig. 7

Osteogenic differentiation of canine adipose tissue-derived MSCs. a–d Small black crystalline structures were observed at 3, 7, 14, and 21 days after osteogenic differentiation. f Deposited crystals were stained with alizarin red S stain which showed reddish-brown coloration. Negative control shown in panel e where no visible stain was observed. g The number of calcified crystalline structures with incubation time. h The characterization of osteocytes through RT-PCR. Expressions of osteopontin and collagen I, marker genes for osteocytes were compared with undifferentiated cells. We found positive expression for both the marker genes in differentiated cells whereas no expression was observed in undifferentiated cells. GAPDH was used as an internal control. The data represent means ± SD. *p < 0.05 was considered statistically significant

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