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Fig. 8 | The Journal of Basic and Applied Zoology

Fig. 8

From: Derivation, characterization, and in vitro cell regeneration of canine white adipose tissue-derived mesenchymal stem cells obtained from a mesenteric region

Fig. 8

Chondrogenic differentiation of canine adipose tissue-derived MSCs. a–d Morphological changes observed at 3, 7, 11, and 14 days after chondrogenic differentiation. f Alcian blue staining for aggrecan bodies during chondrogenic differentiation showed bluish-green coloration. e Negative control where no visible stain was observed. g Size of micromasses represented with bar diagram. h RT-PCR analysis for aggrecan and Sox9, marker genes for chondrocytes. Successful expression of both the marker genes was observed in chondrogenic differentiated cells. Sox9 expression was also observed in undifferentiated cells, although expression was quite low compared to chondrocyte-differentiated cells. GAPDH was used as loading control. The data represent means ± SD. *p < 0.05 was considered statistically significant

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