Folic acid protects and heals gastric mucosa: role of acid output, inflammatory cytokines, angiogenic and growth factors

The Journal of Basic and Applied Zoology

Table 4 Effect of varying doses of folic acid on pyloric ligation induced gastric ulcer: pro-inflammatory and anti-inflammatory cytokines

Group	Treatment	TNF-α (pg/mg protein)	IL-1β (pg/mg protein)	IL-4 (pg/mg protein)	IL-10 (pg/mg protein)
1	Normal saline (1 ml/kg b.w)	15.5 ± 3.5	10.0 ± 3.5	50.5 ± 5.0	70.5 ± 5.0
2	6 h Pyloric Ligation	54.5 ± 5.0^a	45.5 ± 5.0^a	15.7 ± 2.0^a	45.5 ± 5.0^a
3	2FA + 6 h Pyloric Ligation	30.7 ± 2.5^b	25.0 ± 2.5^b	25.6 ± 1.5^b	55.5 ± 2.0^b
4	3FA + 6 h Pyloric Ligation	28.0 ± 5.0^b	25.0 ± 5.0^b	30.5 ± 4.5^b	60.0 ± 2.5^c
5	OMEP + 6 h Pyloric Ligation	25.0 ± 2.0^c	20.0 ± 2.0^c	40.5 ± 1.0^c	65.9 ± 4.0^d

2FA 2 mg/kg Folic acid, 3FA 3 mg/kg Folic acid, OMEP omeprazole
^aP < 0.05, Pyloric ligation versus control group
^bP < 0.05, 2FA + 6 h Pyloric Ligation versus 6 h Pyloric Ligation
^cP < 0.05, Omeprazole + 6 h Pyloric Ligation versus 6 h Pyloric Ligation only. (Mean ± SEM, n = 5)