Carrier RNA is a key factor affecting fully integrated short tandem repeats profiling in challenging forensic samples models

The Journal of Basic and Applied Zoology

Table 1 DNA concentration (ng/µL) and average RFU of alleles A1 & A2 of STR profiles resulted from DNA extracted from 2 types of bloodstain fabrics (F1: balck cotton and F2: denim) using two different extraction methods (EZ and Manual). with and without carrier RNA, at two-time intervals (1st day and 14th day)

Time (d)	Fabric (F)	Methods	Carrier	DNA conc	Average RFU
1	1	EZ	With	6.91 ± 1.10^ab	7730.28 ± 793.52^e
	1	EZ	Without	1.81 ± 0.28^a	5152.50 ± 473.81^d
	1	Manual	With	28.45 ± 8.25^cd	483.36 ± 29.19^a
	1	Manual	Without	36.61 ± 10.31^d	792.89 ± 51.72^a
	2	EZ	With	8.08 ± 1.81^ab	5948.63 ± 1263.08^d
	2	EZ	Without	3.73 ± 1.09^ab	4194.53 ± 702.01^cd
	2	Manual	With	24.16 ± 5.47^cd	2254.53 ± 199.95^ab
	2	Manu al	Without	26.82 ± 8.10^cd	2729.61 ± 709.00^bc
14	1	EZ	With	3.64 ± 1.03^ab	6001.54 ± 707.93^d
	1	EZ	Without	1.29 ± 0.41^a	4431.41 ± 779.89^cd
	1	Manual	With	14.59 ± 3.83^bc	405.71 ± 73.12^a
	1	Manual	Without	16.54 ± 3.02^bc	465.71 ± 138.38^a
	2	EZ	With	3.18 ± 0.87^ab	4304.73 ± 783.08^cd
	2	EZ	Without	1.81 ± 0.28^a	1252.60 ± 251.76^ab
	2	Manual	With	17.70 ± 4.34^bc	733.91 ± 75.66^a
	2	Manual	Without	13.52 ± 4.93^bc	767.39 ± 27.14^a

In the same column, means marked with the same superscript letters are insignificantly different (P > 0.05), whereas those marked with different ones are significantly different (P < 0.05)