Table 2 Comparison of average height peak ( RFU *10³) for each locus of STR profile of DNA extracted from two bloodstain fabrics, black cotton (F1) and denim (F2) using EZ1 extraction method and organic method at 1st day and 14th day

Amel

Time

1

9.17 ± 1.13^C

7.71 ± 1.44^C

0.46 ± 0.01^A

2.92 ± 0.86^B

7.76 ± 1.11^C

6.95 ± 0.77^C

0.80 ± 0.10^A

3.45 ± 0.60^B

14

7.20 ± 1.18^C

5.52 ± 0.87^BC

0.72 ± 0.25^A

0.61 ± 0.04^A*

4.13 ± 1.07^B*

1.04 ± 0.42^A*

0.78 ± 0.26^A

0.41 ± 0.11^A*

TH01

Time

1

8.20 ± 0.98^D

7.95 ± 1.35^CD

0.97 ± 0.11^A

3.14 ± 0.54^B

5.53 ± 0.53^BC

7.25 ± 0.38^CD

1.33 ± 0.16^A

4.73 ± 1.22^B

14

8.00 ± 0.39^C

5.90 ± 0.89^B

0.71 ± 0.10^A

1.30 ± 0.27^A*

5.98 ± 1.36^B

2.38 ± 0.69^A*

0.58 ± 0.16^A*

0.90 ± 0.17^A*

D3S1358

Time

1

10.43 ± 0.59^E

7.18 ± 1.15^D

0.48 ± 0.07^A

2.91 ± 0.55^BC

7.56 ± 1.01^D

4.70 ± 0.64^C

0.71 ± 0.14^A

3.68 ± 1.11^C

14

8.01 ± 0.94^C*

5.66 ± 1.48^B

0.63 ± 0.19^A

0.93 ± 0.22^A*

5.62 ± 0.31^B

4.95 ± 0.46^B

0.32 ± 0.03^A*

1.14 ± 0.39^A*

VWA

Time

1

11.03 ± 1.76^D

8.32 ± 2.29^CD

0.58 ± 0.14^A

2.80 ± 0.79^AB

5.94 ± 1.17^BC

5.54 ± 2.33^BC

0.97 ± 0.30^A

3.18 ± 0.82^AB

14

8.83 ± 1.59^B

7.03 ± 2.30^B

0.52 ± 0.19^A

0.84 ± 0.27^A*

6.04 ± 2.19^B

1.49 ± 0.42^A*

0.33 ± 0.07^A

0.81 ± 0.27^A*

D21S11

Time

1

9.71 ± 1.24^D

7.52 ± 1.29^CD

0.49 ± 0.10^A

2.28 ± 0.28^B

6.52 ± 0.51^C

3.90 ± 1.54^B

0.71 ± 0.10^A

1.89 ± 0.38^AB

14

8.23 ± 0.98^C

4.84 ± 1.21^B*

0.30 ± 0.07^A

0.83 ± 0.13^A*

5.33 ± 1.14^B

1.30 ± 0.13^A*

0.42 ± 0.19^A

0.79 ± 0.19^A*

TPOX

Time

1

6.40 ± 1.09^D

6.25 ± 1.37^D

0.54 ± 0.08^A

2.51 ± 0.25^AB

3.87 ± 0.72^BC

4.84 ± 0.53^CD

0.76 ± 0.24^A

2.99 ± 0.47^BC

14

4.49 ± 1.05^B

3.91 ± 1.08^B*

0.58 ± 0.15^A

1.17 ± 0.41^A*

3.59 ± 0.69^B

0.65 ± 0.14^A*

0.30 ± 0.04^A

0.43 ± 0.11^A*

D7S820

Time

1

9.49 ± 1.47^D

7.16 ± 1.98^CD

0.61 ± 0.11^A

2.73 ± 0.72^AB

7.77 ± 1.53^CD

5.43 ± 1.83^BC

1.10 ± 0.34^A

3.32 ± 0.45^AB

14

8.76 ± 1.38^B

5.98 ± 1.96^B

0.26 ± 0.02^A*

0.90 ± 0.38^A*

6.79 ± 1.99^B

1.46 ± 0.21^A*

0.34 ± 0.07^A*

0.50 ± 0.02^A*

D19S433

Time

1

5.44 ± 0.84^C

5.35 ± 0.95^C

0.53 ± 0.11^A

2.27 ± 0.31^AB

3.66 ± 0.76^BC

2.31 ± 0.64^AB

1.77 ± 0.92^AB

2.43 ± 0.35^AB

14

6.83 ± 0.44^D

2.64 ± 0.48^BC*

0.50 ± 0.05^A

0.91 ± 0.17^AB*

4.06 ± 1.48^C

1.69 ± 0.55^AB

0.42 ± 0.14^A

0.87 ± 0.25^AB*

D5S818

Time

1

11.35 ± .61^D

7.56 ± 1.92^C

0.42 ± 0.05^A

2.22 ± 0.43^AB

8.29 ± 1.14^CD

5.00 ± 1.30^BC

0.67 ± 0.13^A

1.98 ± .75^AB

14

8.88 ± 1.73^B

6.28 ± 0.92^B

0.26 ± 0.06^A

1.07 ± 0.17^A*

6.57 ± 2.38^B

1.64 ± 0.30^A*

0.14 ± 0.03^A*

1.08 ± 0.23^A

D2S1338

Time

1

3.94 ± 054^C

2.63 ± 0.69^B

0.66 ± 0.08^A

1.50 ± 0.46^AB

2.48 ± 0.31^B

1.84 ± 0.12^AB

0.73 ± 0.16^A

1.70 ± .42^AB

14

2.59 ± .50^C*

2.27 ± 0.60^BC

0.35 ± 0.04^A*

0.88 ± 0.18^A

2.12 ± 0.73^BC

0.89 ± 0.03^A

0.68 ± 0.05^A

1.33 ± 0.19^AB

D16S539

Time

1

7.21 ± 0.65^D

5.11 ± 0.82^C

0.60 ± 0.09^A

1.73 ± 0.28^AB

5.80 ± .14^CD

2.90 ± 0.79^B

0.62 ± 0.12^A

1.51 ± .29^AB

14

4.31 ± .65^D*

3.37 ± .91^CD*

0.43 ± 0.13^A

1.13 ± 0.24^AB

3.00 ± .78^CD*

2.38 ± 0.81^BC

0.48 ± 0.13^A

0.85 ± .28^AB

CSF1PO

Time

1

5.31 ± 0.91^D

4.07 ± 0.83^CD

0.41 ± 0.08^A

1.45 ± 0.37^AB

3.53 ± 0.37^C

2.74 ± 0.34^BC

0.53 ± 0.87^A

1.80 ± 0.44^AB

14

3.55 ± 0.68^C*

2.63 ± 0.53^C

0.31 ± 0.18^A

0.70 ± 0.80^AB*

3.03 ± 0.80^C

1.99 ± 0.82^BC

0.40 ± 0.01^AB

0.75 ± 0.20^AB

D13S317

Time

1

6.78 ± 1.16^C

7.26 ± 1.25^C

0.41 ± 0.06^A

1.68 ± 0.29^A

4.34 ± 0.78^B

4.13 ± 1.05^B

0.74 ± 0.09^A

1.20 ± 0.28^A

14

3.72 ± 0.64^B*

2.81 ± 0.97^B*

0.38 ± 0.17^A

0.71 ± 0.09^A

3.04 ± 0.94^B

0.66 ± 0.09^A*

0.45 ± 0.08^A

0.82 ± 0.26^A

FGA

Time

1

7.94 ± 0.91^B

7.98 ± 1.39^B

1.20 ± 0.82^A

1.83 ± 0.45^A

6.30 ± 0.75^B

5.38 ± 0.86^B

0.97 ± 0.27^A

1.96 ± 0.72^A

14

6.99 ± 1.28^D

4.13 ± 0.94^BC*

0.21 ± 0.06^A

0.70 ± 0.11^A

5.39 ± 1.26^CD

2.39 ± 1.06^AB*

0.32 ± 0.06^A*

0.63 ± 0.15^A

D18S51

Time

1

6.35 ± 1.03^D

5.62 ± 1.43^CD

0.31 ± 0.04^A

1.40 ± 0.32^AB

4.89 ± 0.94^CD

3.51 ± 0.38^BC

0.71 ± 0.83^A

1.70 ± 0.44^AB

14

4.45 ± 0.83^C*

3.38 ± 0.86^BC*

0.30 ± 0.07^A

0.55 ± 0.13^A*

3.77 ± 0.67^C

1.91 ± 0.76^AB

0.29 ± 0.09^A*

0.61 ± 0.10^A*

D8S1179Z

Time

1

4.79 ± 0.51^DE

5.28 ± 0.76^E

0.20 ± 0.04^A

1.43 ± 0.45^AB

3.55 ± 0.35^CD

3.53 ± 0.44^CD

0.63 ± 0.06^A

2.31 ± 0.60^BC

14

3.83 ± 0.64^B

2.68 ± 0.50^B*

0.22 ± 0.06^A

0.38 ± 0.06^A*

3.25 ± 0.53^B

1.34 ± 0.58^A*

0.30 ± 0.04^A*

0.44 ± 0.09^A*