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Table 4 Allele dropouts from profiles using two extraction methods (EZ1 and organic), “with and without using carrier RNA” from two types of fabrics (F1 and F2), at two storage times (1st and 14th day)

From: Carrier RNA is a key factor affecting fully integrated short tandem repeats profiling in challenging forensic samples models

PHR % less than 60

Fabric 1

Fabric 2

EZ

Manual

EZ

Manual

With carrier

Without carrier

With carrier

Without carrier

With carrier

Without carrier

With carrier

Without carrier

Volunteer 1

1st day

     

FGA(42%)

Amelognin(42%)

 
        

D19S433 (33%)

 
 

14th day

  

D7S820 (50%)

  

CSF1PO(40%), D8S1179 (50%)

D21S11 (50%)

Amelognin (50%) D21S11 (35%)

    

CSF1PO (40%)

  

D5S818 (40%)

 

FGA (43%)

Volunteer 2

1st day

 

D19S433 (54%)

D2S1338 (22%)

D3S1358 (40%), D5S818 (50)

   

D13S317 (43%)

    

D16S539 (40%)

D2S1338 (37%), CSF (49%)

    
    

D8S1179 (50%)

D18S51 (44%)

    
 

14th day

D8S1179 (49%)

 

D19S433 (42%)

D21S11 (50%), D2S1338 (52%)

D8S119 (49%)

D21S11 (0%)

D19S433 (42%)

Amel(0%), D21S11 (58%), TPOX

    

D8S1179 (57%)

D13S317 (45%), FGA(52%)

  

D5S1179 (57%)

(0%), D19S433 (0%), CSF1PO(0%),13S317 (47%, D18S51 (0%), D21S11 (50%), D5S818 (52%), D135317 (45%)

Volunteer 3

1st day

  

Amelognin(46%)

D16S539 (44%), D8S1179

 

D21S11 (57%)

VWA(26%), D5S818 (59%)

D16S539 (28%), CSF1PO (33%)

    

TH01 (49%)

(53%) D2S1338 (52%)

 

TPOX (58%)

D16S539 (29%), CSF1PO(24%), D13S317 (37%)

D13S317 (20%)

 

14th day

  

TH01 (51%)

D16S539 (57%)

D5S818 (50%)

  

TH01 (52%), D16S539

    

(30%)

  

D21S11 (50%)

D8S1179 (51%)

D13S317 (37%)

    

TPOX(29%), D7S820 (38%)

  

TPOX(33%)

  

Volunteer 4

1st day

  

Amel.(50%), D5S818 (29%)

     
    

D16S539 (51%), D8S1179 (0%)

     
 

14th day

  

D16S539 (59%)

   

TH01 (37%), D3S1358 (51%)

TH01 (53%), D3S1358 (56%), D16S539 (18%).CSF1PO(57%), D13S317 (0%), D18S51 (0%

    

D18S51 (40%)

   

D21S11 (0%), D19S433 (0%)

 
        

D2S1338 (0%), D16S539 (32%) D18S51 (31%)