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Table 6 The in-vitro effect of technical abamectin, chlorfenapyr, and pyridaben on AChE, ATPase, ACP, ALP, GABA-T, CaE, and GST activities isolated from the adult females of two spotted spider mites T. urticae

From: Toxicity, joint action effect, and enzymatic assays of abamectin, chlorfenapyr, and pyridaben against the two-spotted spider mite Tetranychus urticae

Enzyme

Acaricide

I50a (mg/L)

95% confidence limits

Slopeb ± SE

Interceptc ± SE

(χ2)d

Lower

Upper

AChE

Abamectin

8.62

2.99

17.80

0.86 ± 0.06

− 0.81 ± 0.11

23.07

 

Chlorfenapyr

578.09

276.40

1609.90

0.30 ± 0.04

− 0.80 ± 0.09

4.16

 

Pyridaben

140.74

57.70

388.41

0.46 ± 0.05

− 0.99 ± 0.10

14.44

ATPase

Abamectin

0.46

0.17

0.93

0.72 ± 0.08

0.24 ± 0.11

6.24

 

Chlorfenapyr

13.86

5.34

28.04

0.61 ± 0.05

− 0.69 ± 0.10

13.42

 

Pyridaben

8.11

0.89

17.36

0.65 ± 0.05

− 0.51 ± 0.10

29.04

ACP

Abamectin

0.008

0.001

0.06

0.53 ± 0.10

1.10 ± 0.14

0.38

 

Chlorfenapyr

0.095

0.01

0.36

0.42 ± 0.06

0.423 ± 0.10

4.25

 

Pyridaben

0.042

0.65

3.16

0.70 ± 0.11

0.21 ± 0.05

3.58

ALP

Abamectin

0.061

0.005

0.25

0.33 ± 0.09

1.16 ± 0.14

0.52

 

Chlorfenapyr

17.67

7.93

32.95

0.94 ± 0.06

1.17 ± 0.12

21.41

 

Pyridaben

208.12

98.76

560.22

0.76 ± 0.05

− 1.75 ± 0.13

29.51

GABA-T

Abamectin

0.42

0.13

0.92

0.62 ± 0.07

0.24 ± 0.12

9.34

 

Chlorfenapyr

6.41

0.36

23.99

0.39 ± 0.05

− 0.32 ± 0.09

19.74

 

Pyridaben

19.80

10.19

32.24

1.34 ± 0.09

− 1.74 ± 0.15

21.59

CaE

Abamectin

29.99

14.848

54.73

0.85 ± 0.06

− 1.26 ± 0.11

18.48

 

Chlorfenapyr

91.68

61.94

136.10

0.55 ± 0.05

− 1.08 ± 0.10

8.49

 

Pyridaben

11.09

2.39

28.87

0.58 ± 0.05

− 0.61 ± 0.09

23.07

GST

Abamectin

0.043

0.003

0.209

0.41 ± 0.06

0.56 ± 0.11

2.87

 

Chlorfenapyr

82.82

66.47

102.47

1.19 ± 0.08

− 2.28 ± 0.16

4.71

 

Pyridaben

67.67

45.36

98.95

1.33 ± 0.09

− 2.43 ± 0.18

10.34

  1. aHalf maximal inhibitory concentration
  2. bSlope of the concentration-inhibition regression line ± standard error
  3. cThe y-intercept of the regression line ± SE
  4. dChi-square goodness of fit test