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Table 6 The pairwise comparison result of the upper lethal temperature data in Table 3 by two-way ANOVA and the Tukey test, showing intra-breed variation in the thermal tolerance plasticity of mulberry silkworm (Bombyx mori L.)

From: Effect of different lethal temperature assay methods on thermal tolerance plasticity of three different breeds of mulberry silkworm (Bombyx mori L.)

Breeds

Variable

D1

D2

D3

p-value

S1

S2

S3

S1

S2

S3

S1

S2

S3

Day

Stress

DS1

F1

ULT50

42.8 ± 0.227b

40.5 ± 0.217d

38 ± 0.213 fg

43.8 ± 0.244a

41.3 ± 0.186cd

38.9 ± 0.214ef

42.2 ± 0.189bc

39.4 ± 0.237e

37.6 ± 0.152g

 < 0.001

 < 0.001

0.37

 

ULT25

46 ± 0.113b

43.7 ± 0.199d

40.6 ± 0.242fg

47 ± 0.0549a

44.7 ± 0.177c

41.3 ± 0.235f

45.6 ± 0.152bc

42.6 ± 0.26e

40 ± 0.17g

 < 0.001

 < 0.001

0.191

BV

ULT50

39.9 ± 0.213ab

37.1 ± 0.205cd

36.3 ± 0.207de

40.8 ± 0.232a

37.8 ± 0.178c

36.7 ± 0.204d

39.2 ± 0.18b

36.9 ± 0.229cd

35.4 ± 0.146e

 < 0.001

 < 0.001

0.313

 

ULT25

43.8 ± 0.221ab

39.9 ± 0.311cd

38.6 ± 0.315de

44.8 ± 0.202a

40.6 ± 0.273c

39.2 ± 0.31cd

43.3 ± 0.224b

39.7 ± 0.362cd

37.6 ± 0.238e

 < 0.001

 < 0.001

0.56

NP

ULT50

41.2 ± 0.22b

38.9 ± 0.209c

37.1 ± 0.209d

42.6 ± 0.262a

40.4 ± 0.184b

38.4 ± 0.21c

40.4 ± 0.183b

38.3 ± 0.231c

36.3 ± 0.145d

 < 0.001

 < 0.001

0.988

 

ULT25

44.5 ± 0.178ab

42.1 ± 0.241c

39.6 ± 0.274e

45.3 ± 0.108a

43.6 ± 0.196b

40.9 ± 0.248d

44.1 ± 0.199b

41.6 ± 0.291cd

38.8 ± 0.198e

 < 0.001

 < 0.001

0.317

  1. Analysis done on upper lethal temperature data obtained from two-way ramping assay method. Values are means ± SEM, n = 3 per treatment group. a−eMeans in a row without a common superscript letter differ (p < 0.05) as analysed by two-way ANOVA and the Tukey test. 1D S = Day Stress interaction effect