Fig. 5

Photomicrographs of paraffin sections stained with Crossmon's trichrome technique illustrated the protective effect of curcumin (Cur) on diclofenac sodium (DS) induced renal damage in rat. A The kidney of control (Ctrl) group showed normal collagen contents in glomeruli (G) in the continuous renal tubular basement membrane (arrow) and in the continuous basement membrane of the parietal layer of the Bowman’s capsule (arrowhead). B The kidney of DS group showed a less collagen contents in glomeruli (G) in the thin disrupted renal tubular basement membrane (arrow) and in the thin disrupted basement membrane of the parietal layer of the Bowman’s capsule (arrowhead). C The kidney of diclofenac sodium + curcumin (DS + Cur) group showed restoration of the normal collagen contents in glomeruli (G) in the continuous renal tubular basement membrane (arrow) and in the continuous basement membrane of the parietal layer of the Bowman’s capsule (arrowhead). Original magnification X400, scale bar = 50 μm. D Histogram showed that the size of glomerular vessels significantly increased in DS group compared to the control group, and oral supplementation of Cur restored the normal size of the glomerular vessels. E Histogram showed that the size of the renal corpuscles significantly decreased in DS group compared to the control group, and oral supplementation of Cur restored the normal size of the renal corpuscles. F Histogram showed that the size of the glomerulus significantly decreased in DS group compared to the control group, and oral supplementation of Cur restored the normal size of the glomerulus. Results are expressed as mean ± SEM (6 mice/group). Data are analyzed by one-way ANOVA followed by Duncan posttest. ^aindicates significant difference between control and DS groups at p < 0.05; ^bindicates significant difference between DS and DS + Cur groups at p < 0.05; ^cindicates significant difference between control and DS + Cur groups at p < 0.05