Fig. 5

Photomicrographs of liver sections showing the effect of GEN on KET-induced DNA strand breaks (TUNEL assay) and increased in P53 protein expression. In liver section of control rats some cells are positive for TUNEL staining (a) and P53 immunoreactivity (b), the hepatocyte nuclei and cytosol stained brown (arrows). Liver sections of KET-treated rats showing extensive TUNEL-positive cells (c), and P53-positive cells (d), (arrows). Group of rats treated with GEN + KET had nearly normal appearance of hepatocytes with a marked reduction in TUNEL-positive cells (e) and P53-positive cells (f). Sections were counterstained with hematoxylin (X 200). Columns represent number of TUNEL- and P53-positive cells as a percentage of total area (5 scope field count for five animals in each group), and values are expressed as mean ± SEM. Significance was determined by one-way analysis of variance followed by Dennett’s t test:. a and b indicate significant differences relative to control and KET groups, respectively