Assessment ameliorative role of fenugreek seeds and germinated fenugreek seeds on pancreatic and testicular gentamicin toxicity of male Swiss albino mice

The Journal of Basic and Applied Zoology

Table 1 Masson’s trichrome protocol

Staining reagent and rinses	Time	Catalog number
Weigert's Iron Hematoxylin working solution	10 min	HT107*
Running tap water, warm	10 min
Biebrich scarlet-acid fuchsin solution	10–15 min	HT151*
DIH₂O	Rinse
Phosphomolybdic—phosphotungstic Acid solution	15 min	HT153*
DIH₂O Rinse	Brief rinse
aniline blue solution	10 min	B8563*
1% acetic acid solution	5 min	45,754*
DIH₂O	Rinse
Sections dehydrated, cleared and coverslipped