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The Journal of Basic and Applied Zoology
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Analysis of different bioactive compounds in the tissue of the epigeic earthworm, Eisenia fetida

The Journal of Basic and Applied Zoology volume 85, Article number: 28 (2024) Cite this article

Abstract

Background

Eisenia fetida is the epigeic earthworm renowned for organic waste management in vermitechnology. The medicinal properties of earthworm biomass is gaining much more importance in extracting various biomolecules. Therefore, the present study was carried out to analyze the bioactive compounds of Eisenia fetida by using gas chromatography and mass spectroscopy analysis of four different solvent extract and highlighting their biological activities.

Results

The analysis showed the presence of 17, 22, 21 and 18 bioactive compounds in chloroform, ethyl acetate, methanol and distilled water solvent extract, respectively. Each compound were analyzed based on their peak number, R-time and Base m/z values. The molecular formula, molecular weight, compound nature, their structure and biological activities were tabulated.

Conclusion

The presence of a wide range of bioactive compounds in the epigeic earthworm, Eisenia fetida, justifies the pharmacological use in curing various diseases.

Background

Our prehistoric ancestors sought different natural compounds to cure various ills and diseases so as to improve and enrich their lives, most of these compounds were derived from either plants or animals. Research on traditional medicines has been vital in search for organic pharmaceutical compounds. Animal-based medicines have also been extracted and isolated from different parts of the animal body, from their products (secretions and excrements) or from materials they produce like cocoons and compost in earthworm. A total of 252 essential compounds have been selected by World Health Organization (WHO), of which only 8.7% are from animal origin used as medicine (Costa-Neto, 2005). For almost 4000 years, China has a history of research into medicinal uses of earthworm.

Earthworms are well-known invertebrates, are used extensively as folk fare medicines and claim their successful use in treatment of cancer, ulcer, inflammation, diarrhea, fever, dysentery, tooth ache, etc. (Edwards & Bohlen, 1996). Various studies have shown that earthworms exhibit anti-pyretic, anti-septic, detoxifying, diuretic, anti-hypertensive, anti-allergic, anti-asthmatic, spermatocidal, anti-oxidative, anti-microbial, anti-cancer, anti-ulcer and anti-inflammatory activity (Cooper & Roch, 1984; Cooper et al., 2004; Hori et al., 1974). High protein content of earthworm makes it a desired supplementary food material to a wide range of animals such as fish, amphibians, reptiles, birds and mammals (Edwards & Bohlen, 1996). The presence of plant and animal growth-promoting amino acids and other substances showed the therapeutic effects (Edwards & Bohlen, 1996). Presently, there is an increasing demand and interest in naturally produced medicines, accompanied by modern laboratory investigations into pharmacological properties of bioactive ingredients and their ability to treat various diseases (Smita, 2021). Further it encourages looking for other molecules of curative value.

In recent years, zootherapy (the science of treating various ailments by using therapeutic materials obtained from animals) is gaining valuable importance as it is potentially safe drugs to use. Therefore, pharmaceutical industries are much interested and are trying to use these bioactive compounds and other metabolites as potent agents in treatment of many diseases (David & Gordon, 2020). Many studies revealed that primary and secondary metabolites are used in the treatment of chronic as well as infectious diseases (Kesava & Usha, 2016). In recent years, GC–MS has become the key tool for profiling secondary metabolites which is widely used in the separation and identification of complex components, as it has high resolution of GC and high sensitivity of mass spectrometry. Hence, the present study is focused on identification of different bioactive compounds through GC–MS technique from the epigeic earthworm, Eisenia fetida, using different solvent system like distilled water, chloroform, methanol and ethyl acetate.

Methods

  1. a.

    Selection and collection of earthworm: Epigeic earthworm, Eisenia fetida species, was selected based on their mass multiplication rate in vermiculture and was collected from the stock culture maintained in vermitechnology laboratory, Department of Zoology, Karnatak University, Dharwad, as it is voracious feeder and breeder continuously and can withstand a wide range of abiotic factors.

  2. b.

    Preparation of sample powder: Around thirty sexually matured healthy Eisenia fetida earthworms were collected and washed them in distilled water and the surface debris were removed with the help of blotting paper. The cleaned earthworms were sundried for about seven days. The dried biomass was ground to get fine powder with the help of mortar and pistil.

  3. c.

    Sample extract: 2.5 g of powdered sample was used and subjected to extraction with chloroform (40 ml), ethyl acetate (40 ml), methanol (40 ml) and distilled water (40 ml) solvents.

  4. d.

    GC–MS analysis: Gas chromatography–mass spectroscopy analysis was carried out to evaluate various metabolites and bioactive compounds present in the powdered sample, the epigeic earthworm, Eisenia fetida, in four different solvent extracts. The sample was analyzed on GC 2010 with split injection mode and linear velocity flow control mode having 800C of column oven temperature with 65.0 kPa of pressure. The GC program was performed on GC–MS–QP 2010 with 2200C Ion source temperature and 2800C of interface temperature. The solvent cut time was 6.50 min with relative type of detector gain mode, and the detector gain is 1.03 kV + 0.20 kV at 1000 threshold. The start time was 7.00 min, and the end time was 50.00 min with scan ACQ mode. The event time is for 0.50 s with 1000 scan speed.

  5. e.

    Identification of components: Various bioactive compounds are identified based on the retention time (R-time). Interpretation of mass spectrum of GC–MS was done by using the database of the National Institute Standard and Technology (NIST). The data reported in the present study are obtained from various online repositories, websites like MedChem Express, PubChem, ChEBI, ChemSpider, Smolecule, Cymitquimicq and Chemical Book. Based on this, the molecular weight, molecular formula, their structure, nature of compounds and their biological activity were tabulated in the results.

Results

The GC–MS profiling of powdered sample of Eisenia fetida in four different solvent extracts (chloroform, ethyl acetate, methanol and distilled water) gave a wide range of bioactive compounds. The GC–MS chromatogram of chloroform extract showed the peaks indicating the presence of 17 bioactive compounds and is presented in Fig. 1. Table 1 indicates the details of identified bioactive compounds such as name of the compound along with its peak number, R-time, base m/z, compound nature, chemical formula and molecular weight, whereas the structure of compounds and their biological activities are presented in Table 2. Similarly, Figs. 2, 3 and 4 present the GC–MS chromatogram of Eisenia fetida which showed the peaks indicating the presence of 22, 21 and 18 different bioactive compounds in ethyl acetate, methanol and distilled water extract, respectively. Tables 3, 4 and 5 present the details of the bioactive compounds such as compound’s name with its peak number, R-time, base m/z, compound nature, chemical formula and molecular weight, whereas the structure of respective compounds and their biological activities are presented in Tables 6, 7 and 8 with respect to ethyl acetate, methanol and distilled water extracts, respectively.

Fig. 1
figure 1

Chromatogram of GC–MS in chloroform extract of the epigeic earthworm, Eisenia fetida

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Table 1 Details of bioactive compounds analyzed in chloroform extract of the epigeic earthworm, Eisenia fetida, through GC–MS
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Table 2 Structure and bioactivity of the compounds obtained in the chloroform extract of the epigeic earthworm, Eisenia fetida, through GC–MS
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Fig. 2
figure 2

Chromatogram of GC–MS in ethyl acetate extract of the epigeic earthworm, Eisenia fetida

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Fig. 3
figure 3

Chromatogram of GC–MS in methanol extract of the epigeic earthworm, Eisenia fetida

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Fig. 4
figure 4

Chromatogram of GC–MS in distilled water extract of the epigeic earthworm, Eisenia fetida

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Table 3 Details of bioactive compounds in ethyl acetate extract of the epigeic earthworm, Eisenia fetida, through GC–MS
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Table 4 Structure and bioactivity of the compounds in the ethyl acetate extract of the epigeic earthworm, Eisenia fetida, through GC–MS
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Table 5 Details of bioactive compounds in methanol extract of the epigeic earthworm, Eisenia fetida, through GC–MS
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Table 6 Structure and bioactivity of the compounds in the methanol extract of the epigeic earthworm, Eisenia fetida, through GC–MS
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Table 7 Details of bioactive compounds in distilled water extract of the epigeic earthworm, Eisenia fetida, through GC–MS
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Table 8 Structure and bioactivity of the compounds in the distilled water extract of the epigeic earthworm, Eisenia fetida, through GC–MS
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GC–MS analysis of different extracts of the epigeic earthworm, Eisenia fetida, showed various bioactive compounds of pharmaceutical importance. There are in total 78 different bioactive compounds identified from four solvent extracts (chloroform, ethyl acetate, methanol and distilled water), which are chemically alkanes, esters, phenol, alkenes, fatty alcohol, carboxylic acid, quinoline, sulfur allotrope, fluorides, alkyl iodides, salt, alkyl benzene, dicarboxylic acid, aromatic compounds, phytosterol, acyclic monosaturated diterpene alcohol, halides, heterobicyclic compounds, cyclicalkanes, lactum and liquid alkane in nature. Out of 78 compounds identified, the biological activities of 23 compounds are not yet reported. The presence of all these bioactive compounds some or other way showed various pharmaceutical properties. Majority of the compounds obtained in the present study are chemically alkane in nature, exhibiting different biological activities like anti-fungal, anti-bacterial, anti-cancer, anti-pyretic, anti-helminthic, anti-inflammatory, etc. biological activities. Sulfurous acid, pentadecyl 2-propyl ester is chemically ester in nature, and exhibits anti-oxidant, anti-microbial and anti-inflammatory activities (Patil & Deshmukh, 2015). 2-Propenoic acid, pentadecyl ester is also chemically ester in nature helps in hydroxylation of liver enzymes, promotes hair growth, and inhibits the production of uric acid and arachidonic acid in the human body (Shunmugapriya et al., 2017). (E)-Phytol, which is chemically phytosterol in nature, exhibits anti-histomal, anti-nociceptive, anti-oxidant, anti-inflammatory, anti-allergic and anti-microbial activities (de Josué et al., 2014). 3,7,11,15-Tetramethyl-2-hexadecen-1-ol, which is chemically acyclic monosaturated diterpene alcohol in nature, exhibits anti-microbial and anti-inflammatory activities (Rajeswari et al., 2012). The aromatic compound, phenol, 3,5-bis(1,1-dimethylethyl)-, which exhibits anti-oxidant and anti-bacterial activity (Rizvi et al., 2014). The fatty alcohol compound, 2-octyldodecan-1-ol, also exhibits anti-inflammatory, anti-oxidant and anti-microbial activities (Cymitquimicq.com). The dodecane, 1-iodo which is chemically alkyl iodide in nature exhibits anti-diabetic activity (Mahmood et al., 2020). Quinoline, 1,2-dihydro-2,2,4-trimethyl- is chemically quinoline in nature exhibits anti-oxidant property which is also used in styrene–butadiene and nitrile–butadiene rubber and latex (Chemical Book). The sulfur allotrope, octathiocane, exhibits anti-cancer, anti-oxidants and anti-microbial activities (Smolecule). The fatty alcohol, celidoniol, deoxy-, exhibits anti-bacterial (Köse et al., 2016) and anti-inflammatory activity (Zakariaa et al., 2014), which helps in chemical communication in Anopheles stephensi mosquito (Brei et al., 2004) and acts as pheromone of Orgyia leucostigma (Grant et al., 1987). The anti-inflammatory activity is also reported in undecane which is chemically liquid alkane in nature and heptadecyl acetate which is chemically ester in nature (PubChem). 2-Piperidinone which is chemically lactum in nature exhibits anti-microbial activity (Dawood et al., 2019). The acyclic alkane, hexane, 3,3-dimethyl-, has been used in the production of phytochemical compound effective in the removal of heavy metals (Ghorbani et al., 2022). The carboxylic acid, hydrocinnamic acid, exhibits anti-oxidant (Razzaghi-Asl et al., 2013), anti-inflammatory (Nagasaka et al., 2007) and anti-microbial activities (Taofiq et al., 2017). Cyclopentane, 1,1,3-trimethyl- is a alicyclic hydrocarbon and exhibits anti-cancer activity (Alaqeela et al., 2022). The anti-cancer, anti-microbial, anti-diabetic, anti-convulsant, anti-inflammatory, anti-viral and anti-tubercular activities are also reported in benzothiazole, 2-(2-hydroxyethylthio)-, which is a heterobicyclic compound (Ruhi & Nadeem, 2013).

Discussions

Many of these bioactive compounds obtained in the present study through GC–MS are commonly reported in various medicinal plants, which are pharmacologically important. Some of the bioactive compounds like hexadecane, tetradecane, eicosane, nonadecane, phytol and tetracosane are identified in the present study which are also identified in the leaf extract of Waltheria indica Linn. exhibiting similar pharmacological activities (Prabhanna & Jayaraj, 2017). Indra et al., (2018) identified several bioactive compounds in Datura starmonium of which docosane, 1,2-benzenedicarboxylic acid, nonadecane and eicosane are identified in the present study with similar biological activity. Tetratriaconitane, pentadecane and quinoline are also identified in the present work which are present in the fenugreek seed oil with similar biological activity reported by Sweeta et al. (2019). Delicia and Shyam (2008) reported the diverse bioactive compounds in the Bombyx mori gut, of which celidoniol deoxy, 2-bromotetradecane, tetracosane, pentadecane, nonadecane and eicosane were also noticed in the Eisenia fetida extracts of the present study exhibiting similar pharmacological activities. Many such GC–MS analyses of phytochemicals have been focused on plant extracts, and very few studies are available on animal tissue extracts. GC–MS analysis to investigate anthelminthic activity using Corallocarpus epigaeus extracts using four different organic solvents against Pheretima postuma earthworm was reported by Kalpesh and Priya (2020) where different bioactive compounds in treating helminthiasis were identified. GC–MS-based approach to measure the response of earthworm to the exposure of carbofuran in the soil was reported by Mohana et al. (2013).

Thus, various chemical compounds obtained from the epigeic earthworm, Eisenia fetida, in different solvent extracts exhibit a wide range of medicinal/biological properties, which can be life savior and can be used in preparation of new drugs to treat a wide variety of diseases in pharmacological industries.

Conclusion

GC–MS analysis of the powdered extracts of the epigeic earthworm, Eisenia fetida, in four different solvent extracts revealed the presence of various biologically active compounds, which are having important medicinal value. In total, 78 compounds were obtained from four different solvent extracts, out of which 17, 22, 21 and 18 bioactive compounds were analyzed in chloroform, ethyl acetate, methanol and distilled water, respectively.

Maximum compounds were obtained from ethyl acetate (22) followed by methanol (21), distilled water (18) and chloroform (17) extracts. As per the literature, the compounds have shown anti-fungal, anti-bacterial, anti-cancer, anti-pyretic, anti-helminthic, anti-inflammatory, anti-viral, anti-allergic, anti-HIV, anti-malarial, anti-tuberculosis, anti-diabetic, anti-convulsant, anti-chistomal, anti-nociceptive, anti-oxidant properties.

Hence, based on the results of the present study, it can be concluded that the epigeic earthworm, Eisenia fetida, has a potent source of bioactive compounds that can be used in pharmacological activities. Further studies are also essential to isolate, characterize and purify particular active compounds responsible for particular therapeutic activities.

Online repositories/websites used

  • National Institute Standard and Technology (NIST)

  • PubChem

  • CheBI

  • MedChem Express

  • Chemspider

  • Smolecule

  • Chemical Book

  • Cymitquimic.com

Availability of data and materials

All data analyzed during the present study are included in this article. Please contact authors for data.

References

  • Akpuaka, A., Ekwenchi, M. M., Dashak, D. A., & Dildar, A. (2013). Biological activities of characterized isolates of n-Hexane extract of Azadirachta indica A. Juss (Neem) leaves. Natural Sciences, 11(5), 141–147.

    Google Scholar 

  • Alaqeela, N. K., Almalki, W. H., Binothmon, N., Alijadani, M., Al-Dhuayan, I. S., Alnamshan, M. M., Almulhim, J., Alqosaibi, A. I., Ajmal, M. R., Alammari, D. M., & Tarique, M. (2022). The inhibitory and anti-cancer properties of Annona squamosa L. seed extracts. Brazilian Journal of Biology, 82, 1–15. https://doi.org/10.1590/1519-6984:268250

    Article  Google Scholar 

  • Alqasim, A. M. (2013). Annona senegalensis Persoon: A multipurpose shrub, its phytotherapic, phytopharmacological and phytomedicinal uses. International Journal of Science and Technology, 2(12), 862–865.

    Google Scholar 

  • Apoorva, S., Bernard, F. R., & Yogita, S. (2018). Anti-microbial and anti-cancer activity of Setosphaeria monoceras, an endophytic fungus associated with tropical mangrove plant. World Journal of Pharmacy and Pharmaceutical Sciences, 6(6), 1315–1329. https://doi.org/10.20959/wjpps20186-11819

    Article  CAS  Google Scholar 

  • Brei, B., Edman, J. D., Gerade, B., & Clark, J. M. (2004). Relative abundance of two cuticular hydrocarbons indicates whether a mosquito is old enough to transmit malaria parasites. Journal of Medical Entomology, 41(4), 807–809.

    Article  PubMed  CAS  Google Scholar 

  • Choo, C. Y., Chan, K. L., Sam, T. W., Hitotsuyanagi, Y., & Koichi, T. (2001). The cytotoxicity and chemical constituents of the hexane fraction of Typhonium flagelliforme (Araceace). Journal of Ethnopharmcology, 77, 129–131.

    Article  CAS  Google Scholar 

  • Cooper, E. L., Hrzenjak, T. M., & Grdisa, T. (2004). Alternative sources of fibrinolytic, anticoagulative, antimicrobial and anticancer molecules. International Journal of Immunopathology and Pharmacology, 17, 237–244.

    Article  PubMed  CAS  Google Scholar 

  • Cooper, E. L., & Roch, P. (1984). Earthworm leukocyte interactions during early stages of graft rejection. Journal of Experimental Zoology, 232, 67–72.

    Article  PubMed  CAS  Google Scholar 

  • Costa-Neto, E. M. (2005). Animal-based medicines: Biological prospection and the sustainable use of zootherapeutic resources. Anais Da Academia Brasileira De Ciências, 77(1), 33–43.

    Article  PubMed  Google Scholar 

  • Dabin, C., Wesuk, K., & Taesun, P. (2020). Anti-allergic and anti-inflammatory effects of undecane on mast cells and keratinocytes. Molecules, 25(7), 1554. https://doi.org/10.3390/molecules25071554

    Article  CAS  Google Scholar 

  • Dandekar, R., Fegade, B., & Bhaskar, V. H. (2015). GC-MS analysis of phytoconstituents in alcohol extract of Epiphyllum oxypetalum leaves. Journal of Pharmacognosy and Phytochemistry, 4(1), 149–154.

    Google Scholar 

  • David, J. N., & Gordon, M. C. (2020). Natural products as source of new drugs over the nearly four decades from 01/1981 to 09/2019. Journal of Natural Products, 83, 770–803. https://doi.org/10.1021/acs.jnatprod.9601285

    Article  Google Scholar 

  • Dawood, C. H., Al-Bahadily, Falah, H. S., Mazin, A. A. N., & Al-Salnman, H. N. K. (2019). Anti-microbial activity of the compound 2-piperidionone, N-[-4-Bromo-n-butyl]- extracted from Pomogranate peel. Asian Journal of Pharmaceutics, 13(1), 46.

  • de Josué, M., Rosimeire, N. D., Jéssica, P. C., Antonio, L. G. J., Damião, P. D., Rivelilson, M. F., Silmara, M. A., & Pedro, L. S. P. (2014). Phytol, a Diterpene alcohol from chlorophyll, as a drug against neglected tropical disease schistosomiasis mansoni. PLOS Neglected Tropical Disease, 8(1), e2617. https://doi.org/10.1371/journal.pntd.0002617

    Article  CAS  Google Scholar 

  • Delicia, A. B., & Shyam, K. V. (2008). GC-MS analysis of bioactive compounds and antimicrobial activity of Cryptococcus rajasthanensis KY627764 isolated from Bombyx mori gut microflora. International Journal of Advanced Research, 6(3), 525–538. https://doi.org/10.21474/IJAR01/6700

    Article  Google Scholar 

  • Edwards, C. A., & Bohlen, P. J. (1996). Biology and ecology of earthworms (3rd ed.). Chapman and Hall.

    Google Scholar 

  • Geeta, R., Lalita, Y., & Kalidhar, S. B. (2009). Chemical examination of Citrus Sinensis flavedo variety pineapple. Indian Journal of Pharmaceutical Sciences, 71(6), 677–679.

    Article  Google Scholar 

  • Ghorbani, E., Nowruzi, B., Nezhadali, M., & Hekmat, A. (2022). Metal removal capability of two cyanobacterial species in autotrophic and mixotrophic mode of nutrition. BMC Microbiology, 22, 58. https://doi.org/10.1186/s12866-022-02471-8

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Gnanavel, V., & Mary, A. S. (2013). GC–MS analysis of petroleum ether and ethanol leaf extracts from Abrus precatorius Linn. International Journal of Pharmacy and Biological Sciences, 4(3), 37–44.

    CAS  Google Scholar 

  • Grant, G. G., Frech, D., MacDonald, L., Slessor, K. N., & King, G. G. S. (1987). Copulation releaser pheromone in body scales of female white marked tussock moth, Orgyia leucostigma (Lepidoptera: Lymantriidae): Identification and behavioral role. Journal Chemical Ecology, 13, 345–356.

    Article  CAS  Google Scholar 

  • Gumgumjee, N. M., & Hajar, S. A. (2015). Antibacterial activities and GC–MS analysis of phytocomponents of Ehretia abyssinica R. Br. ex fresen. International Journal of Applied Biology and Pharma Technology, 6(2), 236–241.

    Google Scholar 

  • Gurudeeban, S., Sathyavani, K., & Ramanathan, T. (2010). Bitter apple (Citrulus colocynthis): An overview of chemical composition and biomedical potentials. Asian Journal Plant Sciences, 9, 394.

    Article  CAS  Google Scholar 

  • Haval, H. M., & Fuad, O. A. (2022). Microwave assisted extraction and phytochemical profile of Nonea pulmonarioides and its antifungal, antibacterial, and antioxidant activities. Journal of Food Quality, 2022, 5135880. https://doi.org/10.1155/2022/5135880

    Article  CAS  Google Scholar 

  • Holldobler, B., & Wilson, E. O. (1990). The ants (p. 287). Cambridge: Harvard University Press.

    Book  Google Scholar 

  • Hori, M., Kondon, K., Yoshida, T., Konishi, E., & Minami, S. (1974). Studies of anti-pyretic components in the Japanese earthworm. Biochemical Pharmacology, 23, 1582–1590.

    Article  Google Scholar 

  • Hsouna, A. B., Trigie, M., Mansour, R. B., Jarraya, R. M., Damak, M., & Jaoua, S. (2011). Chemical composition, cytotoxicity effect and antimicrobial activity of Ceratoniasilisqua essential oil with preservative effects against listeria inoculated in minced beef meat. International Journal of Food Microbiology, 148(1), 66–72.

    Article  PubMed  Google Scholar 

  • Indra, R., Pallavi, D., Priya, T., Taniya, J., Nishesh, S., & Manish, D. S. (2018). GC–MS analysis of plant leaf extract of Datura stramonium in different solvent system. European Journal of Biomedical and Pharmaceutical Sciences, 5(10), 236–245.

    Google Scholar 

  • Javidnia, K. R., Miri, M. S., Gholami, M., & Khosravi, A. R. (2008). Antimicrobial activity and chemical composition of the essential oils of six Iranian Salvia species. Chemistry of Natural Composition, 44(5), 654–658.

    Article  CAS  Google Scholar 

  • Juan, A., Morales, R., Elena, D., Miriam, A. C., Richard, G. W., & Jorge, F. T. (2009). Thermo-mechanical properties of candelilla wax and dotriacontane organogels in safflower oil. European Journal of Lipid Science and Technology, 111(2), 207–215. https://doi.org/10.1002/ejlt.200810174

    Article  CAS  Google Scholar 

  • Jyothirmayi, N., Prasad, S. H. R. K., & Rao, N. M. (2014). Antibacterial activity and GC–MS analysis of ripened and unripened cv. Amrutapani (Musa x paradisiaca L.) banana extracts. Journal of Medical Sciences and Technology, 3(3), 138–144.

    Google Scholar 

  • Kalpesh, B. I., & Priya, S. K. (2020). In vitro anthelmintic activity and phytochemical characterization of Collacarpus epigaeus (Rottler) Hook. f. tuber from ethyl acetate extracts. Bulletin of National Research Centre, 44(33), 1–10. https://doi.org/10.1186/s42269-020-00286-z

    Article  Google Scholar 

  • Kawuri, R., & Darmayasa, I. B. G. (2019). Bioactive compounds of Streptomyces capoamus as bio-control of bacterial wilt diseases on Banana plant. IOP Conference Series: Earth and Environmental Science, 347, 012054. https://doi.org/10.1088/1755-1315/347/1/012054

    Article  Google Scholar 

  • Kesava, R. B., & Usha, R. G. (2016). GC–MS analysis of volatile components in petroleum ether extracts of Coldenia procumbens Linn. International Journal of Pharmacy and Biosciences, 7(2), 241–245.

    CAS  Google Scholar 

  • Köse, Y. B., Iscan, G., & Demirci, B. (2016). Antimicrobial activity of the essential oils obtained from flowering aerial parts of Centaurea lycopifolia Boiss. et Kotschy and Centaurea cheirolopha (Fenzl) Wagenitz from Turkey. Journal of Essential Oil Bearing Plants, 19(3), 762–768.

    Article  Google Scholar 

  • Mahmood, R., Kayani, W. K., Ahmed, T., Malik, F., Hussain, S., Ashfaq, M., Ali, H., Rubnawaz, S., Green, B. D., Calderwood, D., Kenny, O., Rivera, G. A., Mirza, B., & Rasheed, F. (2020). Assessment of anti-diabetic potential and phytochemical profiling of Rhazya stricta root extracts. BMC Complementary Medicine and Therapies, 20, 293. https://doi.org/10.1186/s12906-020-03035-x

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Mahmoodreza, M., Forough, K., Hossein, T., & Younes, G. (2010). Composition of the essential oil of Rosa damascena mill from South of Iran. Iranian Journal of Psychiatry Sciences Winter, 6(1), 59–62.

    Google Scholar 

  • Marzoqi, A., Hameed, I., & Salah, I. (2015). Analysis of bioactive chemical components of two medicinal plants (Coriandrum sativum and Melia azedarach) leaves using Gas Chromatography-Mass Spectrometry (GC-MS). African Journal of Biotechnology, 14(40), 2812–2830.

    Article  Google Scholar 

  • Mihailovi, V., Vukovi, N., Niforori, N., Soluji, S., Mladenovi, M., MaÅ¡kovic, P., & Milan, S. (2011). Studies on anti-microbial activity and chemical composition of the essential oils and alcoholic extracts of Gentiana asclepiadea L. Journal of Medicinal Plants Research, 5(7), 1164–1174.

    Google Scholar 

  • Mohana, K. R. M., Ratnashekar, Ch., & Prem, N. S. (2013). Gas chromatography–mass spectroscopy based metabolomic approach for optimization and toxicity evaluation of earthworm sub-lethal responses to Carbofuran. PLoS ONE, 8(12), e81077. https://doi.org/10.1371/journal.pone.0081077

    Article  CAS  Google Scholar 

  • Mustapha, N. A., & Runner, R. T. M. (2016). GC–MS analysis of and preliminary antimicrobial activity of Albizia adianthifolia (Schumach) and Pterocarpus angolensis (DC). Medicines, 3(3), 1–9. https://doi.org/10.3390/medicines3010003

    Article  CAS  Google Scholar 

  • Naemi, F., Asghari, G., & Yousofi, H. (2014). Chemical composition of essential oil and anti-trichomonas activity of leaf, stem, and flower of Rheum ribes Linn. extracts. Avicenna Journal of Phytomedicine, 4(3), 191–199.

    PubMed  PubMed Central  CAS  Google Scholar 

  • Nagasaka, R., Chotimarkorn, C., Shafiqul, I. M., Hori, M., Ozaki, H., & Ushio, H. (2007). Anti-inflammatory effects of hydrocinnamic acid derivatives. Biochemistry and Biophysics Research Communication, 358, 615–619. https://doi.org/10.1016/j.bbrc.2007.04.178

    Article  CAS  Google Scholar 

  • Neda, M. D., Biljana, B., Marina, S., & Natasa, S. (2004). Antimicrobial and antioxidant activities of Melissa officinalis Linn. (Lamiaceae) essential oil. Journal of Agricultural and Food Chemistry, 52, 2485–2489.

    Article  Google Scholar 

  • Ombito, O. J., Matasyoh, C. J., & Vulule, M. J. (2014). Chemical composition and larvicidal activity of Zanthoxylem gilletii essential oil against Anopheles gambie. African Journal of Biotechnology, 13(21), 2175–2180.

    Article  Google Scholar 

  • Patil, U. S., & Deshmukh, O. S. (2015). GC–MS analysis of phytochemicals in the aqueous extract of Cyclea peltata. (Lam) Hook. F. & Thomson. International Journal of Scientific Research, 4(11), 350–351.

    Google Scholar 

  • Paul, A. V. N., Singh, S., & Singh, A. K. (2002). Kairomonal effect of some saturated hydrocarbons on the egg parasitoids, Trichogramma brasiliensis (Ashmead) and Trichogramma exiguum (Hymenoptera: Trichogrammatidae). Journal of Applied Entomology, 126, 409–416.

    Article  CAS  Google Scholar 

  • Prabhanna, B., & Jayaraj, M. (2017). GC–MS analysis of bioactive compounds from ethanolic leaf extracts of Waltheria indica Linn. and their pharmacological activities. International Journal of Pharmaceutical Science and Research, 9(5), 2005–2010. https://doi.org/10.13040/IJPSR.0975-8232.9(5).2005-10

    Article  Google Scholar 

  • Prathapa, R. M., Kavya, B., Rama, R. V., Shantha, T. R., Kishore Kumar, R., Venkateshwarlu, G., & Rahmathulla, R. (2015). Therapeutic uses of flowers-leads from traditional system of medicine. International Journal of Herbal Medicine, 3(3), 12–20.

    Google Scholar 

  • Rajeswari, G., Murugan, M., & Mohan, V. R. (2012). GC–MS Analysis of bioactive components of Hugonia mystax L. (Linaceae). Research Journal of Pharmaceutical and Bio Chemical Science, 3(4), 301–308.

    CAS  Google Scholar 

  • Razzaghi-Asl, N., Garrido, J., Khazraci, H., Borges, F., & Firuzi, O. (2013). Anti-oxidant properties of Hydrocinnamic acid: A review of structure- activity relationships. Current Medical Chemistry, 20, 4450. https://doi.org/10.2174/09298673113209990141

    Article  CAS  Google Scholar 

  • Rizvi, S. M., Shakil, S., Zeeshan, M., Khan, M. S., Shaikh, S., Biswas, D., Ahmad, A., & Kamal, M. A. (2014). An enzoinformatics study targeting polo-like kinases-1 enzyme: comparative assessment of anticancer potential of compounds isolated from leaves of Ageratum houstonianum. Pharmacognosy Magazine, 10(37), S14–S21. https://doi.org/10.4103/0973-1296.127333

    Article  PubMed  PubMed Central  Google Scholar 

  • Ruhi, A., & Nadeem, S. (2013). Biological aspects of emerging Benzothiazoles: a short review. Journal of Chemistry, 2013, 345198. https://doi.org/10.1155/2013/345198

    Article  CAS  Google Scholar 

  • Shaikh, J. U., Darren, G., & Evelin, T. (2012). Evaluation of cytotoxic activity of patriscabratine, tetracosane and various flavonoides isolated from the Bangladeshi medicinal plant Acrostichum aureum. Pharmaceutical Biology, 50(10), 1276–1280.

    Article  Google Scholar 

  • Shunmugapriya, K., Vennila, P., Thirukkumar, S., & Ilamaran, M. (2017). Identification of bioactive components in Moringa oleifera fruit by GC-MS. Journal of Pharmacognosy and Phytochemistry, 6(3), 748–751.

    CAS  Google Scholar 

  • Smita, G. B. (2021). Medicinal plants and its pharmacological values. Natural Medicinal Plants, 12, 217–228. https://doi.org/10.5772/intechopen.99848

    Article  Google Scholar 

  • Song, Y. W., Lim, Y., & Cho, S. K. (2018). 2, 4-Di-tert-butylphenol, a potential HDAC6 inhibitor, induces senescence and mitotic catastrophe in human gastric adenocarcinoma AGS cells. Biochimica et Biophysica Acta Molecular Cell Research, 1865(5), 675–683. https://doi.org/10.1016/j.bbamcr.2018.02.003

    Article  PubMed  CAS  Google Scholar 

  • Sowmya, S., Perumal, P., & Velliyur, K. G. (2015). Chromatographic and spectrophotometric analysis of bioactive compounds from Cayratia trifolia (Linn.) stem. International Journal of Pharmacy and Pharmaceutical Sciences, 8(6), 56–64.

    Google Scholar 

  • Sweeta, A., Abdurahman, N. H., Yunus, R. M., Alara, O. R., & Abayomi, O. O. (2019). Extraction, characterization and antioxidant activity of fenugreek (Trigonella-Foenum Graecum) seed oil. Materials Science for Energy Technologies, 2, 349–355.

    Article  Google Scholar 

  • Taofiq, O., Gonzalez-Parama’s, A., Barreiro, M., & Ferreira, I. (2017). Hydrocinnamic acid and their derivatives: cosmecutical significance, challenges and future perspectives: A review. Molecules, 22, 281. https://doi.org/10.3390/molecules22020281

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Thirunavukkarasu, K., Rajkumar, P., Selvaraj, S., & Kumaresan, S. (2016). GC–MS analysis of Gymnema sylvestre leaves methanolic extract for antidiabetic and anticancer drug identification. Journal of Chemical and Pharmaceutical Sciences, 9(2), 1011–1013.

    CAS  Google Scholar 

  • Uddin, S. J., Grice, D., & Tiralongo, E. (2012). Evaluation of cytotoxic activity of patriscabratine, tetracosane and various flavonoids isolated from the Bangladeshi medicinal plant Acrostichum aureum. Pharmaceutical Biology, 50(10), 1276–1280.

    Article  PubMed  CAS  Google Scholar 

  • Yogeswari, S., Ramalakshmi, S., Neelavathy, R., & Muthumary, J. (2012). Identification and comparative studies of different volatile fractions from Monochaetia kansensis by GCMS. Global Journal of Pharmacology, 6(2), 65–71.

    Google Scholar 

  • Zakariaa, M. B., Ilhama, Z., & Muhamad, N. A. (2014). Anti-inflammatory activity of Calophyllum inophyllum fruits extracts. Procedia Chemistry, 13, 218–222.

    Article  Google Scholar 

  • Zhao, L., Shuge, T., Wen, E., & Halmuart, U. (2017). An ethnopharmacological study of aromatic Uyghur medicinal plants in Xinjiang, China. Pharmaceutical Biology, 55(1), 1114–1130.

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The authors are extremely thankful for the guidance of Dr. Arun K. Shettar, Research Scientist, at Cytxon Biosolutions Pvt Ltd. in analyzing the GC–MS results.

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The research did not receive any specific grant from funding agencies in the public, commercial or not-for-profit sectors.

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  1. Department of Zoology, Karnatak University, Dharwad, Karnataka, 580 003, India

    Aishwarya Shetty & Pulikeshi M. Biradar

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  1. Aishwarya Shetty
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AS carried out the experiment, analyzed the data and prepared the manuscript, and PMB corrected and finalized the manuscript.

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Correspondence to Pulikeshi M. Biradar.

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Shetty, A., Biradar, P.M. Analysis of different bioactive compounds in the tissue of the epigeic earthworm, Eisenia fetida. JoBAZ 85, 28 (2024). https://doi.org/10.1186/s41936-024-00381-x

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