Impact of hiking trails on the diversity of flower-visiting insects in Wadi Telah, St. Katherine protectorate, Egypt

A hiking trail is one of the major recreational activities that have critical impacts on wildlife. Wadi Telah in Saint Katherine Protectorate (South Sinai, Egypt) (N 28.568539 and E 33.932816) is an important spot for biodiversity. It was surveyed for spatial variation in the diversity of flower-visiting insects at different distances from the hiking trail. The current study aimed at assessing the impact of some anthropogenic activities along the main hiking trail passing through W. Telah on the diversity of flower-visiting insects. Field monitoring of flower visitors across the hiking trail passing through W. Telah was carried out at different distances (~ 0, 20, 40, and 60 m) along both sides of the hiking trail. As a control, monitoring was performed at 120 m from the hiking trail. A total of 542 individuals belonging to 34 species of flower visitors, 16 families, from three orders were recorded within the study localities in W. Telah. Floral resources in Wadi Telah can be a limiting factor for the populations of flower visitors. There was a significant difference in the distribution of the flower visitors’ species richness among different distances from the hiking trail in W. Telah. Species richness and abundance of flower visitors and their associated floral resources were significantly positively correlated to the distance from the hiking trail. There were no significant differences in the diversity of flower-visiting insects among different distances from the trail, perhaps due to their mobility. The present study showed that the hiking trail had a negative impact on flower visitors and their associated floral resources in Wadi Telah. The investigation on how different biodiversity elements respond to hiking trails allows greater insight for protectorate managers. The study can contribute to effective management decisions for current and future protectorates.


Introduction
St. Katherine Protectorate (SKP) is a distinctive area for biological diversity with high scientific and economic value. It includes the highest mountains in Egypt, which uphold special ecosystems, with a distinctive faunal and floral diversity (Baha El Din, 2006) and a relatively high occurrence of endemic species (SKP-M.P, 2003).
Wadi Telah (1370-1608 m a.s.l.) (N 28.568539 and E 33.932816) is one of the most important biodiversity rich spots in SKP area. A major hiking trail about 4 km long and about (1-2 m) wide pass through the rich spots in W. Telah and is used by local Bedouins and tourists during their visits to the area. It is an informal trail created by the trampling actions of the visitors.
Protected areas permit a separation of the direct effect of anthropogenic activities on biodiversity from those of other environmental variables (Sinclair, Mduma, & Arcese, 2002). Accordingly, the protected area presents ideal systems for evaluating the impact of human activities (Souza & Prevedello, 2020). Protected areas are substantial for long-term conservation of overexploited plants. Also, the frequency and density of native species are higher inside protected areas (Souza & Prevedello, 2020). Also, protected areas are generally efficient at preserving habitats for species and thus saving suitable conditions for the occurrence and maintenance of many species (Geldmann et al., 2013). SKP faces several threats to its conservation status (Fouda et al., 2006). The protectorate faces some global threats such as climate change and tourism, while others arise from local necessities and socioeconomic issues (e.g., the overharvesting of wild plants) (Grainger & Gilbert, 2008).
Nature-based tourism and recreation activities such as hiking, the viewing of wildlife, and cycling close to or within protected areas might have negative impacts on animal populations and biodiversity within protected areas (Huhta & Sulkava, 2014). There are apparent direct impacts of recreation activities such as damage from trampling or clearing of vegetation for infrastructure (Pickering & Hill, 2007). The impacts on fauna, flora, soil, and water resources are generally most obvious at areas subjected to massive visitor numbers especially hiking trails and campsites (Tomczyk, 2011). A hiking trail is a critical recreational activity that has serious impacts on ecosystems (Queiroz, Ventura, & Silva, 2014). Hiking trail may displace animals from otherwise suitable habitat; also, animals may change their activity patterns according to interactions with recreationists (Reilly, Tobler, Sonderegger, & Beier, 2017). The human disturbance had a great effect on the animal foraging percentage in the daytime activity budget, which increased as the day proceed and at greater distances from the closest hiking trail (Pęksa & Ciach, 2018).
Many insects visit plant flowers, to obtain pollen, nectar, or feed on other flower parts. Many of these interactions result in pollination. Globally, several studies on the flower-visiting insects, especially insect pollinators are endangered and in decline over the world (Winfree, Bartomeus, & Cariveau, 2011). The diversity and abundance of pollinators are negatively affected by several stresses like climate change, habitat destruction, fragmentation, and the unavailability of floral resources (Shakeel et al., 2019). Insect pollinators have mutual relationships with flowering plants to gain nectar and pollen (Arenas & Farina, 2012). Decreased pollinators' diversity and abundance may lead to a decline in plant species diversity (Biesmeijer et al., 2006). Pollinators provide several ecological services including the following: maintain a healthy ecosystem, increase the genetic diversity of plants, secure plant reproduction, and increase the plant yields, such as the number of pods, seeds, seed weights per plant, and seed germination (Siregar, Atmowidi, & Kahono, 2016).
Insect pollinators as are effective bioindicators that can be used to monitor several environmental impacts performed by biological factors such as diseases, parasites, introduced competitors, and predators as well as by physical and chemical factors, particularly habitat modification and pesticides (Kevan, 1999;Siddig, Ellison, Ochs, Villar-Leeman, & Lau, 2016). Habitat modification and fragmentation brought by human activities are key drivers of change to plant-pollination interactions (Stout, 2014;Winfree, Aguilar, Vázquez, Lebuhn, & Aizen, 2009). Recent evidence indicated that diversity and abundance of highly mobile pollinator species can be affected by the landscape assembly and configuration around the site in which they are being monitored (Kennedy et al., 2013;Stout, 2014).
Recent evidence showed that the impact of the hiking trail is less harmful than a road impact, but even a narrow hiking trail could fragment the habitat of small invertebrates (Mader, 1984). Also, several types of research assessed local-scale impacts of trails by comparing effects at increasing distances from a trail and against undisturbed conditions. Species richness is used to assess the anthropogenic impact such as habitat changes on biodiversity (Barragan, Moreno, Escobar, Halffter, & Navarrete, 2011;Michelsen, McDevitt, & Coelho, 2014). Accordingly, the present study is designed for identifying and quantifying of flower visitors' species within Wadi Telah. The study is also aimed at assessing the impact of the hiking trail passing through Wadi Telah on the diversity of flower-visiting insects.

Study area
St. Katherine Protectorate extends over the entire mountain massif of South Sinai with an area of 4350 km 2 (Fig. 1) and it is characterized by the highest elevations in Egypt and a dense Wadi system (SKP-M.P, 2003). SKP is the coolest area in Sinai due to its high elevation (1500-2641 m above sea level). It was declared as a protected area in 1996 due to its biological and cultural significance (SKP-M.P, 2003). SKP is characterized by its arid climate with little rains in winter which reflects a unique flora and fauna diversity (Semida, 2006). SKP is one of the richest spots for flora diversity in the Middle East, from which~472 plant species have been reported (Fayed & Shaltout, 2004). Twenty-six endemic plant species were found in SKP which comprise 42.6% of Egypt endemic plant species (Moustafa, Abd El-Wahab, & Shaker, 2001).
Wadi Telah is considered a long and narrow valley about 6 km long, with steep slopes of high boulder fields that drop to the valley bed. It is surrounded from both sides by Mt. El-Serw (2150 m high) to the east and extension of Gebel Abas Basha at the southwest (N 28.58723, E 33.92017). It has several Bedouin gardens including different types of fruit trees such as apricot, almond, apple, and date palm, so it is considered a highly diverse wadi. The dominant plants are Achillea fragrantissima, Origanum syriacum, Capparis sinaica, Peganum harmala, Asclepias sinaica, Alkanna orientalis, and Teucrium polium (Rashad, Atwa, & El Sharqawy, 2012).

Monitoring and identification of biodiversity elements in W. Telah
A bimonthly field survey started in October 2018 to October 2019. Each monitoring session spanned over 1 week. Field monitoring of flower-visiting insects' species across the hiking trail passing through W. Telah was carried out at different distances (~0, 20, 40, and 60 m) along both sides of the hiking trail (Figs. 2 and 3). As a control, monitoring was conducted at 120 m from the hiking trail. Fifty sampling points that cover the different distances from the hiking trail were chosen for collecting data.

Monitoring and identification of flower-visiting insects
Monitoring of flower-visiting insects was done using transect technique, by direct observations within the study area. According to Ballantyne and Pickering (2015), several studies used observational methods to collect quantitative data, such as transect surveys and point sampling. The total number of insects' visits to the plant flowers in the 30-min focal watch and the species richness was calculated. All samples are collected using a net trap. The identification of flower-visiting insects was done by using different keys and books to reach the family level, genus level, and species level. Insects were sorted into taxa and then grouped into morpho-species based on visual characteristics. Different species of flower visitors were identified using the study of Larsen (1990), Mcgavin (1992), Goulet and Huber (1993), Gilbert and Zalat (2008), and Rashad et al. (2012).

Data analysis
Spatial variation of flower-visiting insects' species in W. Telah was measured using species richness (S) and species evenness (E). Alpha diversity (α-diversity) within the study area was measured using Simpson's diversity index (D) and the Shannon-Wiener index of diversity (H). One-way ANOVA test was used to compare the distribution of the flower visitors' species richness among different distances from the hiking trail in W.  Telah. Spearman correlation test was used to measure the relation between flower-visiting insects' species richness and abundance, and distance from the hiking trail for assessing the impact of the hiking trail on flower-visiting insects within W. Telah. The chi-square test of goodness-of-fit was also used to determine whether there was a significant difference in the flower visitors' diversity among different distances from the hiking trail in W. Telah. SPSS computer package (ver.18) was used to carry out the statistical analysis.

Flower visitors' diversity and abundance in Wadi Telah
A total of 542 individuals belonging to 34 species of flower-visitors, 16 families, from four orders were recorded from October 2018 to October 2019. Flower-visiting insects' species recorded in the study sites of W. Telah was given in Table 1. Most species belonged to family Lycaenidae and family Pieridae from order Lepidoptera, family Dermestidae from order Coleoptera, and family Apidae from order Hymenoptera. No significant difference in the distribution of flower visitors' diversity among different distances from the hiking trail (~0, 20, 40, 60, and 120 m) in W. Telah was reported (χ 2 = 0.71, df = 4, P = 0.9498) ( Table 2). Although the highest species richness was recorded at distance 120 m from the hiking trail (S = 28), the highest diversity was recorded at distance 40 m from the hiking trail (D = 0.937 ± 0.009 and H = 2.907) due to the species evenness (E) recorded at distance 40 m from the hiking trail (E = 0.882) was more than that recorded at distance 120 m from the hiking trail (E = 0.865), while lowest diversity was recorded at distance 0 m from the hiking trail (D = 0.806 ± 0.051 and H = 1.311) with species richness equal to four and species evenness equal to 0.946.

Spatial distribution of the flower visitors' species richness among different distances from the hiking trail in W. Telah
There was a significant difference in the distribution of the flower visitors' species richness among different distances from the hiking trail in W. Telah (F (4, 45) = 11.206, P < 0.01). Distance 120 m from the hiking trail showed the highest mean number of the flower visitors' species richness 11.5, as compared to 3, 3.3, 3.5, and 5.9 at distances 0, 20, 40, and 60 m from the hiking trail, respectively (Fig. 4).
Post hoc test according to Tukey's method is performed to test multiple comparisons between different distances from the hiking trail in W. Telah in the distribution of the flower visitors' species richness. There was a significant difference between distances 120 and 0, 20, 40, and 60 m from the trail equal to 8.5, 8.1, 7.9, and 5.59, respectively (P < 0.05).

Impact of hiking trail disturbance on the flower visitors' species richness and abundance distribution within the study localities in W. Telah
Flower visitors' species richness along the hiking trail was significantly positively correlated to the distance from the hiking trail (rs = 0.556, P < 0.01). The highest flower visitors' species richness at one sampling point within the study localities in W. Telah was recorded at 120 m from the hiking trail (S = 16) (Fig. 5). The abundance of flower visitors along the hiking trail was significantly positively correlated to the distance from the hiking trail (rs = 0. 323, P < 0.01). The highest flower visitors' abundance was recorded at 20 m from the hiking trail (10 individuals for one species) (Fig. 6).

Flora diversity and abundance in Wadi Telah
A total of 280 individuals belonging to 19 plant species and 13 families were recorded in W. Telah (Table 3), aiming at measuring the species richness, abundance, diversity, and density of plant species. Spatial variation in diversity and flora' species richness among the different distances from the trail is shown in Table 4.
The highest number of plant species belonged to family Lamiaceae. However, the highest number of individuals collected belonged to family Tetradiclidaceae. Total flora diversity in W. Telah according to Simpson's diversity index (D) was 0.965 ± 0.006 and according to the Shannon-Wiener index of diversity (H) was 2.521 with species evenness (E) equal to 0.856. Peganum harmala L., Achillea fragrantissima Sch.Bip., Capparis spinosa L., Deverra tortuosa DC., Asclepias sinaica (Boiss.) Muschler., Fagonia mollis Delile., and Stachys aegyptiaca Pers. represented the most frequent species in the study localities in W. Telah.
No significant difference in the distribution of floral diversity among different distances from the hiking trail (~0, 20, 40, 60, and 120 m) in W. Telah was reported (χ 2 = 0.47, df = 4, P = 0.9764) ( Table 4). According to Simpson's diversity index (D), the highest diversity was recorded at distance 120 m from the hiking trail (D = 0.931 ± 0.014) with species richness (S) equal to 16, while the lowest diversity was recorded at distance 20 m from the hiking trail (D = 0.787 ± 0.059) with species richness equal to 9. According to the Shannon-Wiener diversity index (H), the highest diversity was recorded at distance 40 m from the hiking trail (H = 2.577) with species evenness (E) equal to 0.892, while the lowest diversity was recorded at distance 0 m from the hiking trail (H = 1.475) with species evenness (E) equal to 0.917. The highest plant density was recorded distance 120 m from the hiking trail (density = 11.5), while the lowest plant density was recorded distance 20 m from the hiking trail. The highest plant species richness was recorded at distance 40 m from the hiking trail.
Spatial distribution of the plant species richness among different distances from the hiking trail in W. Telah There was a significant difference in the distribution of the plant species richness among different distances from the hiking trail in W. Telah (F (4, 45) = 9.263, P < 0.01). Distance 120 m from the hiking trail showed the highest mean number of the plant species richness 8.5, Fig. 4 The spatial pattern of the flower visitors' species richness distribution among different distances from the hiking trail in W. Telah as compared to 2.5, 2.33, 3.1, and 4.7 at distances 0, 20, 40, and 60 m from the hiking trail, respectively (Fig. 7). The post hoc test according to Tukey's method is performed to test multiple comparisons between different distances from the hiking trail in W. Telah in the distribution of the plant species richness. There was a significant difference between distances 120 and 0, 20, 40, and 60 m from the trail equal to 6, 6.1, 5.3, and 3.7, respectively (P < 0.05).

Impact of hiking trail disturbance on the plant species richness and abundance distribution within the study localities in W. Telah
The plant species richness along the hiking trail was significantly positively correlated to the distance from the hiking trail (rs = 0.563, P < 0.01). The highest plant species richness at one sampling point within the study localities in W. Telah was recorded at 120 m from the hiking trail (S = 14) (Fig. 8). The abundance of plant Fig. 6 The relationship between the distance from the hiking trail and flower visitors' abundance within the study localities in W. Telah species along the hiking trail was significantly positively correlated to the distance from the hiking trail (rs = 0. 344, P < 0.01). The highest plant abundance was recorded at 40 m from the hiking trail (21 individuals for one species) (Fig. 9).

Discussion
One of the challenges facing conservation biology was the increase in anthropogenic effects on biodiversity hotspots (Zafra-Calvo, Rodriguez, & Lobo, 2010). Anthropogenic activates such as land-use alteration, pathogens, pesticides, and climate change can have critical impacts on insect pollinators and the pollination services they provide (Cariveau & Winfree, 2015). Globally, pollinators are threatened by land-use change including degradation of seminatural habitats and several studies are concerned that insect pollinators are in decline (Stein et al., 2018;Winfree et al., 2009). Hiking trails and nature-based tourism might have negative impacts on faunal biodiversity within protected areas (Huhta & Sulkava, 2014). Unplanned visitor-created informal trails are existed in several protected areas and perform further fragmentation effects by expanding the impact into relatively undisturbed habitats (Leung, Pickering, & Cole, 2012). Also, recent studies have focused on the effects of anthropogenic activities such as habitat modification and fragmentation on pollinator species richness and diversity, and community composition of pollinators (Eckert et al., 2010;Stanley & Stout, 2013;Stout, 2014). The result showed that the diversity of flower-visiting insects in W. Telah was high. This agrees with the findings of Zalat et al. (2001) who reported that high altitude region of SKP had the highest-flying insect diversity. Also, the results reported 34 species of flower visitors are belonging to four insect orders (Coleoptera, Diptera, Hymenoptera, and Lepidoptera) in W. Telah, and 112 species are belonging to five insect orders (Coleoptera, Diptera, Hemiptera, Hymenoptera, and Lepidoptera) that were recorded before in SKP by (Norfolk & Dathe, 2019). The present study also indicated that there were no significant differences in the diversity of flowervisiting insects among different distances from the trail, perhaps due to their mobility. This result is consistent with the previous study that reveals that the mobility of the flying insects among Katherine, Gebal, and Gharaba systems was the reason for not recording a significant difference in the diversity of flying insects among different systems (Zalat et al., 2001).
The present study showed that the sampling points at distance 40 m from the hiking trail had the highest diversity of flower visitors. This might be attributed to the high number of plant species in a flowering stage recorded mainly at that distance. This is agreeing with the previous studies showed that the provision of further floral resources increases both pollinators' diversity and abundance (Norfolk, Eichhorn, & Gilbert, 2014b;Norfolk, Eichhorn, & Gilbert, 2016). Also, a recent study reported that within a wadi having numerous flowers on a plant secured more insect visits (Gilbert et al., 1996). Vegetation density and species richness positively affect Fig. 8 The relationship between the distance from the hiking trail and the plants' species richness within the study localities in W. Telah Fig. 9 The relationship between the distance from the hiking trail and the plant abundance within the study localities in W. Telah Kamel The Journal of Basic and Applied Zoology (2020) 81:52 Page 10 of 13 pollinator activity (Hegland & Boeke, 2006). Floral resources can be considered as a limiting factor for populations of bees (Roulston & Goodell, 2011), syrphid flies (Hegland & Boeke, 2006;Meyer, Jauker, & Steffan-Dewenter, 2009), and Lepidoptera (Ockinger & smith, 2006). The present study showed that the disturbance resulting from the hiking trail had a negative impact on different species of flower visitors and their associated floral resources in Wadi Telah, which is clear from the spatial distribution of species richness of flower visitors and their associated floral resources among different distances from the hiking trail in W. Telah, which is concurred with the view of Ciach, Maślanka, Krzus, and WOJAS (2017) who reported that species richness and abundance of insect pollinators decreases with increased hiking trail disturbances by a human. Similarly, Gray and Jongepier (2012) showed a decrease in insect species diversity with an increase in the human disturbance, when studying the relationship between the level of human disturbance and the diversity of arthropod communities in Kirindy Forest, Western Madagascar.
Furthermore, the results that were consistent with the previous study reveal that disturbance resulting from degradation of seminatural habitats often leads to lowered pollinator abundance and/or diversity (Stein et al., 2018). Also, butterflies and bees both exert strong negative responses to land-use change and extreme land-use causes a strong decrease in species richness and/or abundance (Winfree et al., 2009). Furthermore, habitat fragmentation and loss are the main cause of the decline in bees besides other stresses, including, exposure to parasites, pathogens and pesticides, shortage of floral sources, and the impacts of climate change (Osman & Shebl, 2020). In contrast, the present study does not agree with recent evidence showed that moderate landuse disturbances may maximize the abundance and richness of insect pollinators, including bees (Kessler et al., 2009) and butterflies (Hogsden & Hutchinson, 2004).

Recommendation
The investigation on how different biodiversity elements respond to hiking trails allows greater insight for protectorate managers. The study can contribute to effective management decisions for current and future protectorates. To support the conservation of biodiversity, it is advisable to encourage more researches on the impact of hiking trails and associated human activities on other biodiversity icons such as mammals, reptiles, and birds in SKP. The protectorate management units need to control the usage of informal trails and improve the conservation of plant communities in SKP.