Zamzam water may act as pesticide with and without globe artichoke extract against the cotton mealy bug, Phenacoccus solenopsis

The holy water, Zamzam has many benefits to the human body. Therefore, the present study aims to clear new use of Zamzam water that it can be used as pesticide. In this study, Zamzam water was tested as pesticide alone and with globe artichoke extract and the globe artichoke extract also with tap water against the cotton mealybug, Phenacoccus solenopsis. The obtained results proved that, Zamzam water only has mortality rate approaches the mortality rate of the globe artichoke extract alone. Zamzam water achieved 60% mortality against the adult, P. solenopsis. While 20.000 ppm globe artichoke extract with tap water achieved 70% mortality. Zamzam water, also, improved the effect of globe artichoke extract when diluted with it and the mortality increased to be 86.67% at 15,000 ppm. Likewise, LC50 was 2055.4 ppm with globe artichoke extract with Zamzam water, while it was 13,494.8 ppm with globe artichoke extract without Zamzam water. However, LC90 was 35,791.5 ppm with globe artichoke extract with Zamzam water while it was 48,922.9 ppm with globe artichoke extract without Zamzam water. Also, the chemical analysis of Zamzam water proved the difference in its composition than tap water especially in cations and anions that causes properties like magnetism. Based on the obtained results, the globe artichoke extract with Zamzam water was the most toxic in control of P. solenopsis. This may open great attention to the other uses of the holy water, Zamzam water in pests’ management and encourage more researches about globe artichoke leaves extract and Zamzam water.


Background
Water is a basic need for life and is essential for various physiological functions in human body (Cazier & Gekas, 2001). Although the sources of water are limited, and the existing sources are depleting rapidly, there is a source of water that is called Zamzam which is providing water to billions of people (Khalid et al., 2014;Naeem et al., 1983). Zamzam water is located in the Mecca area, which is a part of Saudi Arabia. Khalid et al., 2014 cleared in their review article that Zamzam water has a strong anti-inflammatory effect and can be used to recover from some diseases. Also, it has analytic action through an indirect effect on endocrine immunology and the growth system of the body (Ali et al., 2009). Artichoke, Cynara cardunculus L. is an ancient herbaceous plant, originating from the Mediterranean area and widely cultivated all over the world. Also, it represents a serious component of the Mediterranean diet and a rich source of bioactive phenolic compounds, minerals, fibres and Page 2 of 6 Abd-Allah The Journal of Basic and Applied Zoology (2022) 83:21 inulin (Lattanzio, 1982;Orlovskaya et al., 2007). Furthermore, the leaves, rich in phenolic compounds (Fratianni et al., 2007;Lattanzio et al., 1989Lattanzio et al., , 1994 are used as herbal medicine since ancient times. Extracts from artichoke have been used for hepatoprotection as diuretic, choleretic, liver-protective, and lipid-lowering agents (Adzet et al., 1987;Gebhardt & Fausel, 1997;Preziosi, 1969). Artichoke leaf extract contains 0.3% flavonoids that expressed as luteolin-7-O-glucoside and 2.5% caffeoylquinic acid expressed as (Indena S.p.A. and chlorogenic acid) (Llorach et al., 2005). The cotton mealybug, Phenacoccus solenopsis Tinsley (Homoptera: Pseudococcidae), has been caused severe damage to cotton in India and Pakistan (Hodgson et al., 2008), and also to many other crops, ornamentals, weeds and medicinal plants (Arif et al., 2009).
Because of problems caused due to huge use of chemical pesticides, many scientists have great attention to natural products to avoid these problems (Abou-Yousef et al., 2010). The aim of this study is to examine the efficiency of Zamzam water only, artichoke leaves extract with and without Zamzam water in managing P. solenopsis.

Methods
Cotton mealybug rearing P. solenopsis was collected from infested okra plants (Abelmoschus esculentus), during fall season, at the field of Aga district, Dakhalia Governorate, Egypt and transferred to the laboratory. Sprouting potato tubers were used as a host plant for the rearing of mealybug and gravid females were inserted on it. Each sprouted potato was infested with an adult female and observed daily (Attia & Ebrahim, 2015). From these reared culture, the newly hatched crawlers were placed on each sprouted potato before being confined in a carton cylindrical box of 8 cm long and 12 cm diameter. Daily examination for morphological changes were recorded and monitored until adult emergence (Attia & Ebrahim, 2015).

Zamzam water
It had brought from Mekka and was used after about one year.

Preparation of plant sample and extraction
Leaves of globe artichoke plant were left to dry at room temperature for about one month then the dried leaves were grinded into fine powder. The powder was soaked in a mixture of methanol, acetone and hexane solvents of equal proportion (1:1:1) in a flask for about one week. After then, the flask was shaked in a shaker and the contents were filtered. Then, the solvents were evaporated under reduced pressure. Finally, the crude extract was weighted and then kept in the deep freezer until use.

Preparing the stock solution of the tested plant extract
Convenient stock concentrations of globe artichoke leaves extract, were prepared on basis of powder weight and volume of the tap water or Zamzam water (w/v) in the presence of tween 80 (0.1%) as emulsifier. The concentrations were kept inside glass stoppered bottles and stored under refrigeration. Four diluted concentrations for each artichoke plant extract were used to draw the LC-P Lines and three replicates were used for each concentration.

Application method
For conducting the experiment, thirty newly emerged adults for each treatment, 10 individuals in each replicate, were placed on okra leaves in each Petri dish. Artichoke extract diluted with Zamzam water had four concentrations, 1000, 5000, 10,000 and 15,000 ppm. While artichoke extract diluted with tap water had concentrations, 5000, 10,000, 15,000 and 20,000 ppm. The concentrations were sprayed on the individuals comparing with control (tap water) and Zamzam water; that had three replicates for each. Mortality was recorded for 7 days after treatment and the mortality percentage was estimated and corrected according to (Abbott, 1925). LC 50 values were determined using probit analysis statistical method of Finney (1971). Sun equation, (1950)

Chemical analysis of Zamzam water and tap water
Chemical analysis of Zamzam water and tap water was conducted in Soil, Water & Environment Research Institute, Mansoura Branch. Analysis was corrected according to Ayers and Westcot (1985). Analysis of electric conductivity was determined using EC meter; while PH was determined by PH meter.

Toxicity effect Efficiency of globe artichoke extract with and without Zamzam water and Zamzam water alone against P. solenopsis
Results in Table 1 and Fig. 1 cleared that, Zamzam water only had high toxic effect on P. solenopsis. This effect was approaching the effect of the extract especially the artichoke without Zamzam water. The total mortality caused due to mealybug treatment by Zamzam water was 60% and this is the same proportion caused due to treatment with 5000 ppm of artichoke extract with Zamzam water and closed to the proportion caused due to treatment with 15,000 and 20,000 ppm artichoke extract without Zamzam water that represented 53.33% and 70% for the two concentrations, respectively. Mortality in control (tap water) was 0%. Also, data in Table 1 represented that mortality after one day of P. solenopsis treated with Zamzam water only was 6.67% and this was the same proportion of P. solenopsis treated with globe artichoke without Zamzam water at 5000 ppm after one and three days, at 10,000 ppm after three and seven days and at 15,000 ppm after seven days of treatment. However, mortality after three day of P. solenopsis treated with Zamzam water only was 30% and this was the same proportion of P. solenopsis treated with globe artichoke with Zamzam water at 5000 ppm after five and seven days, at 10,000 ppm after one day, at 15,000 ppm after seven days and after one day of globe artichoke extract without Zamzam water at 20,000 ppm. Likewise, mortality after five days was of P. solenopsis treated with Zamzam water only 13.33% and this was the same proportion of P. solenopsis treated with 10,000 ppm of globe artichoke extract with Zamzam water after five days and 10,000 ppm of globe artichoke extract without Zamzam water after one day.
As soon as after seven days of treatment with Zamzam water only, mortality was 10%. This proportion was the same proportion of P. solenopsis mortality after three days of treatment with 10,000 ppm of globe artichoke extract with Zamzam water; after five days of 15,000 ppm and after five and seven days of 20,000 ppm of globe artichoke extract without Zamzam water.
Moreover, Table 2 and Fig. 2 showed that, LC 50 was 2055.4 ppm and LC 90 was 35,791.5 ppm for the globe artichoke with Zamzam water. While, LC 50 for the globe artichoke without Zamzam water was 13,494.8 ppm Page 4 of 6 Abd-Allah The Journal of Basic and Applied Zoology (2022) 83:21 and LC 90 was 48,922.9 ppm. These results proved that, Zamzam water improve the efficiency of the globe artichoke extract. Also, slope values indicated that, the globe artichoke extract without Zamzam water had a highest value 2.29 while the slop value of the globe artichoke with Zamzam water was: 1.03. Data also showed that the toxicity index (Ti = 100) 100% for the globe artichoke extract with Zamzam water, while it recorded 15.23% for the globe artichoke extract without Zamzam water.
In addition, The LC 90 /LC 50 confirm the value of this criterion recorded 17.4 and 3.6 for the globe artichoke extract with Zamzam water and the globe artichoke without Zamzam water, respectively. Table 3 demonstrated that, PH value for Zamzam water (Z) was higher than tap water (T) that was 7.40 and

Discussion
Zamzam water that used in management of P. solenopsis was stored for about one year and its properties didn't change. Basem (2011) proved that, the quality of the water did not change for 2 years and there was an excellent agreement among the results of the 30 water samples as well as between the results of the 2 years for the same samples analyzed in 2007 and 2008. Many researches made studies to compare between Zamzam water and magnetic water, so in the present study, the toxic effect of Zamzam water on the pest may be due to the magnetic effect of Zamzam water because Zamzam well lies besides magnetic rocks. Aseel et al. (2016) made a study on the comparative effect between Zamzam water and magnetic water on carcinoid and normal in vivo inside the mice body, the study confirmed that the best and safest therapies in the treatment of mammary gland could be achieved by using Zamzam water, beside that the magnetic water seemed to have a similar but lesser effect on cancer cells. The researchers confirmed that the treatment of water by magnetic zone causing changes in its specifications by making it more absorbing from the cells, after being noticed that water molecules were disassembly much more quick, due to that water consists from high number of cluster molecules (Davis, 2004). This molecule disassembly is accompanied by the hydrogen: oxygen connective. Disassembly causing changing in some water properties likes pH, Surface tention, viscosity and electrical conduction (Alkhazan and Amna, 2010). Also, it plays an important role in treatment of many diseases (Barnstable, 2014), as well as cancer cells because these cells do not live in alkaline environment (Mohammed et al., 2009); all these properties were applied on Zamzam water. Ivan (2011) studied the refraction index of tap water, magnetic water and Zamzam water and proved that Zamzam water had the highest refraction index as soon as more than magnetic water; and added that, the properties of magnetic water approached the properties of Zamzam water then concluded that, Zamzam water may be mainly magnetized due to the nature of strong rocks that surrounded it. Also, Zamzam water improved the efficiency of globe artichoke extract and caused raise in mortality rate against P. solenopsis than globe artichoke extract alone. Hegazy and Fatma (2019) proved that the use of zamzam water and its various concentrations and magnetic water 50% + zamzam water 50% improve the degree of water up take, vase life of survival flowers, fresh weight and recommended the use of zamzam water and magnetic water and its various concentrations for the longevity of cut Rose flowers.
Amalia (2019) conducted the antimicrobial activity of Zamzam water against Salmonella typhi in vitro and proved that zamzam water had antimicrobial activity against S. typhii.
The chemical analysis of Zamzam water comparing with tap water proved that Zamzam water had quality features than tap water. Basem (2011) determined that Zamzam water is alkaline (average pH is 8) with an average Li concentration of 15 μg L (− 1), the alkalinity of Zamzam water and the presence of trace amounts of As and Li may cause the healing power.

Conclusions
Zamzam water improved the properties of globe artichoke extract and achieved higher mortality than globe artichoke extract alone; as soon as Zamzam water alone caused mortality to P. solenopsis. So, Zamzam water has a new use beside its medical use that it can be used as a pesticide or helps in improvement of efficiency of natural pesticides for management of the destructive pest, the cotton mealybug, Phenacoccus solenopsis.