Table 1 Primers list used for Sra-til gene amplification. All primers were ordered from Eurofins MWG/Operon https://www.eurofinsgenomics.eu/en/home.aspx
Primer name | Sequence (5′-3′) | Target |
|---|---|---|
oligodT-T7I | GAGAGAGGATCCAAGTACTAATACGACTCACTATAGGGAGATT24 | cDNA synthesis |
oligodT-T7II | GAGAGAGGATCCAAGTACTAATACGACTCACTATAGG | 3′end |
SL-1 (spliced leader) | GGTTTAATTACCCAAGTTTGAG | 5′start |
tilf1 | CAAAAAAATCCACAAAAATGTGGTG | 3′end with OdT-T7II (partial) |
tilr1 | ATCACCTGTTTTAGTGTTTCTTAC | 5′start with SL-1 (partial) |
tilf0 | ATGAGACTATTTATTAGTTTAC | full length |
tilr0 | TTATGGTCTACTGCCTCTTGGACAT | full length |