Fig. 5

Proteolytic activity of SCF using different substrates assayed using zymogram protease assay. Arrows in red indicate zones of digestion of protein substrates. a Fibrinolytic activity of SCF. b Fibrinogenolytic activity of SCF. c Caseinolytic activity of SCF. a Fibrinolytic activity of SCF (4 μg/ml) was assayed by copolymerizing fibrinogen and thrombin to yield fibrin in the acrylamide gel followed by incubation of the gel in incubation buffer for 1, 2, and 5 h incubation period at 37 °C. Fibrinolytic activity was observed at all three incubation intervals, seen as digestion of the substrate. The figure shows fibrinolysis by SCF after 1 h of incubation. b SCF (4 μg/ml) digested fibrinogen copolymerized with the acrylamide gel within 1 h of incubation at 37 °C. c SCF (4 μg/ml) digested casein present in the polyacrylamide gel seen as destained region of the zymogram gel. Activity was seen within 1 h of incubation at 37 °C