Fig. 5

M3 group liver showing a many necrotic areas (n), bilirubin filled the blood sinusoid and between hepatocytes (↑↑), degeneration of most of the hepatocytes and small dark stain nuclei was observed (↑) (H&E). b Marked extensive necrosis of large area of hepatocytes with dispersion of their contents and areas devoid of hepatocytes (↑). Note: RBCs in the destructed area (hemorrhage) (arrowhead) (H&E). c Polyploid nuclei (red and blue color) (↑), chromatin of few nuclei segregation in their inner membrane and appeared empty (arrowhead) and other hepatocytes with small dark stained nuclei (↑↑). Notice lipofuscin pigments (yellow-brown color) in few Kupffer cells (K) (H&E). d A nuclei showed chromatolysis, B Irregular shape of nuclei, C nuclei enlarged in size with clumped of their chromatin and D hypertrophic of binucleated hepatocytes with pyknotic nuclei (toluidine blue stain). e A Hepatocyte with many of vacuoles differed in size (↑↑), apoptotic cells (arrowhead), and dilated and congested blood sinusoids with a thickened wall (↑). B Hepatocyte contains large vacuole (toluidine blue stain)