Observations | pH 3 | pH 4 | pH 5 | pH 6 | Control |
---|---|---|---|---|---|
Morphological | None as mortality was recorded | Very dark body colouration, slimy mucous secretions on the gills and body surface; peeling and skin erosion, bleeding from the dorsal fins and bending of the caudal fin, some fins became disintegrated | Very dark body colouration, slimy mucous secretions on the gills and body surface; peeling and skin erosion, bleeding from the dorsal fins and bending of the caudal fin, some fins became disintegrated | Normal body colouration except some few dark patches, no morphological abnormalities recorded. | Normal body colouration, no morphological abnormalities seen |
Behavioural | None as mortality was recorded | Erratic swimming, accelerated operculum, gasping, barbells vibration and frequent coming to the surface, feeding impaired Swimming velocity decreased | Erratic swimming, accelerated operculum, gasping, barbells vibration and frequent coming to the surface, feeding impaired, Swimming velocity decreased | No behavioural abnormalities observed. | Normal movement and behavioural responses observed |