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Table 7 Biochemical characterization of Gram-negative isolates from used leech

From: Antibiotic sensitivity and RAPD-PCR studies on cultivable gut bacteria from Indian Medicinal Leech—Hirudinaria granulosa

Sr. no.

Isolates →

TL02

TL03

TL05

TL08

TL13

Name of test ↓

1

Citrate utilization

+ve

-ve

+ve

+ve

+ve

2

Lysine utilization

-ve

-ve

-ve

-ve

-ve

3

Ornithin test

-ve

-ve

-ve

-ve

+ve

4

Urease production

-ve

-ve

+ve

+ve

+ve

5

Phenylalanine utilization

-ve

-ve

-ve

+ve

+ve

6

Nitrate reduction

-ve

-ve

-ve

-ve

-ve

7

H2S test

-ve

-ve

-ve

-ve

+ve

8

Glucose utilization

-ve

-ve

+ve

+ve

+ve

9

Adonitol utilization

-ve

-ve

-ve

-ve

-ve

10

Lactose utilization

-ve

-ve

+ve

-ve

-ve

11

Arabinose utilization

-ve

-ve

+ve

+ve

+ve

12

Sorbitol utilization

-ve

-ve

-ve

-ve

-ve

13

MR test

-ve

+ve

-ve

-ve

-ve

14

VP test

-ve

-ve

-ve

-ve

-ve

15

Oxidase production

+ve

+ve

-ve

+ve

+ve

16

Gelatin hydrolysis

-ve

+ve

+ve

-ve

-ve

17

Starch hydrolysis

+ve

+ve

-ve

-ve

+ve

18

Casein hydrolysis

-ve

-ve

-ve

+ve

+ve

Probable bacteria

P. putida

V. salmonicidaorE. coli(inactive)

Providencia rettgeriorEnt. agglomerans

Burkhoderia gladioli

Citrobacter diversus