Table 8 Biochemical characterization of used leech Gram-positive isolates
Sr. no. | Isolates → | TL01 | TL04 | TL06 | TL07 | TL09 | TL10 | TL11 | TL12 |
|---|---|---|---|---|---|---|---|---|---|
Name of Test ↓ | |||||||||
1 | Malonate utilization | +ve | +ve | +ve | +ve | -ve | -ve | -ve | -ve |
2 | V.P. test | -ve | -ve | -ve | -ve | -ve | +ve | -ve | +ve |
3 | Citrate utilization | +ve | +ve | +ve | +ve | +ve | -ve | +ve | -ve |
4 | ONPG test | -ve | -ve | -ve | -ve | -ve | -ve | -ve | -ve |
5 | Nitrate reduction | -ve | -ve | -ve | -ve | -ve | -ve | -ve | +ve |
6 | Catalase production | +ve | +ve | +ve | +ve | +ve | +ve | -ve | +ve |
7 | Arginine test | +ve | +ve | +ve | +ve | +ve | -ve | -ve | +ve |
8 | Sucrose utilization | -ve | -ve | -ve | -ve | -ve | +ve | -ve | +ve |
9 | Mannitol utilization | -ve | -ve | -ve | -ve | -ve | +ve | -ve | +ve |
10 | Glucose utilization | -ve | +ve | +ve | +ve | -ve | +ve | +ve | +ve |
11 | Arabenose utilization | +ve | -ve | -ve | -ve | -ve | -ve | +ve | -ve |
12 | Thehalose utilization | -ve | -ve | -ve | -ve | -ve | -ve | -ve | -ve |
13 | MR test | -ve | -ve | +ve | -ve | -ve | +ve | -ve | -ve |
14 | Oxidase test | +ve | +ve | -ve | +ve | -ve | +ve | +ve | +ve |
15 | H2S production | -ve | -ve | -ve | -ve | -ve | -ve | -ve | -ve |
16 | Gelatin hydrolysis | -ve | +ve | +ve | +ve | -ve | -ve | +ve | +ve |
17 | Starch hydrolysis | +ve | +ve | +ve | +ve | -ve | +ve | -ve | -ve |
18 | Casein hydrolysis | -ve | -ve | +ve | +ve | -ve | +ve | -ve | -ve |
Probable bacteria | Bacillus megaterium | Bacillus megaterium | Corynebacterium spps. | Flavobacterium spps. | Bacillus spps. | S. cohnii | Bacillus pumilus | Bacillus coagulans | |