Fig. 9

Photomicrographs of H&E-, PAS-, and TNFR2-stained sections of developing skin at E18. The control skin contains Malpighian (M), stratum spinosum (SS), stratum granulosum (SG), stratum cornium (SC), and a well-developed hair follicle (HF) (a). PAS- and TNFR2-stained skin sections revealed localization of carbohydrates and TNFR2 expression in the epidermis (b, c). The skin of GA3-treated embryos showed a thin wrinkled epidermis, a less abundant and disorganized hair follicles with shrinkage of dermal papilla and decrease in PAS and TNFR2 expression in the epidermis (d–f). The skin of olive oil-treated embryo showed H&E and PAS staining similar to control, but high TNFR2 expression (i). The skin of GA3-olive oil-treated embryos showed improvement in the histopathological changes (j–l). Scale bar (a–l) = 10 μm