Influence of fishmeal-replaced diet on nutrient digestibility, digestive enzyme activity, and whole-body fatty acid profile of Indian major carp, Cirrhinus mrigala

The Journal of Basic and Applied Zoology

Table 1 Dietary formulation (g kg⁻¹) and chemical analysis (g kg⁻¹ dry matter) of the experimental diet

Feed ingredients	Dietary treatments
Feed ingredients	Control feed	Test feed
Soya bean meal	200.0	299.1
Groundnut oil cake	350.0	141.5
Mustard oil cake	0.00	236.4
Rice bran	380.0	293.0
Fish meal	50.0	0.00
Linseed oil	0.00	10.0
Vitamin^a and mineral^b Premix	20.0	20.0
Chemical composition
Crude protein	307.4	306.2
Crude lipid	72.4	67.8
Ash	117.5	122.7
Fiber	98.4	101.4
NFE^C	404.3	401.9
Total energy (MJkg⁻¹)	17.12	16.92
Fatty acids
ΣSFA^d	24.61	21.25
ΣMUFA^e	44.82	42.29
ΣPUFA^f n-6	27.16	27.40
18:3 n-3	0.81	3.70
20:5 n-3	0.15	ND
22:6 n-3	0.39	ND
ΣPUFA n-3	1.35	3.70

^aVitamin (IU or g kg⁻¹ premix): retinol palmitate, 50,000 I.U; thiamine, 5; riboflavin, 5; niacin, 25; folic acid, 1; pyridoxine, 5; cyanocobalomin, 5; ascorbic acid, 10; cholecalciferol, 50,000 IU; α-tocopherol, 2.5; menadione, 2; inositol, 25; pantothenic acid, 10; choline chloride, 100; biotin, 0.25
^bMinerals (g kg⁻¹): CaCO3, 336; KH2PO4, 502; MgSO4.7H2O, 162; NaCl, 49.8; Fe(II) gluconate, 10.9; MnSO4.H2O, 3.12; ZnSO4.7H2O, 4.67; CuSO4.5H2O, 0.62; KI, 0.16; CoCl2.6H2O, 0.08; ammonium molybdate, 0.06; NaSeO3, 0.02
^CNitrogen free extract (NFE): 1000- (protein + lipid + fiber + ash)
^dSFA saturated fatty acid
^eMUFA mono unsaturated fatty acid
^fPUFA poly unsaturated fatty acid