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The Journal of Basic and Applied Zoology
Fig. 9 | The Journal of Basic and Applied Zoology

Fig. 9

From: Derivation, characterization, and in vitro cell regeneration of canine white adipose tissue-derived mesenchymal stem cells obtained from a mesenteric region

Fig. 9

Adipogenic differentiation of canine adipose tissue-derived MSCs. a–d Morphological changes were observed after 7, 14, 17, and 21 days of adipogenic differentiation. Formation of lipid droplet was observed in differentiated cells during this period. f Lipid droplets were stained with oil O red stain and e used as a negative control. g Number of lipid droplets with incubation period. h RT-PCR analysis for leptin and FABP4, marker genes for adipocytes. We observed much higher expression of leptin in differentiated cells, whereas FABP4 expression was observed only in adipogenic differentiated cells. GAPDH was used as loading control. The data represent means ± SD. *p < 0.05 was considered statistically significant

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