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Table 1 Composition of the experimental diet in g/100 g dry matter containing various inclusion levels of ascorbic acid and iron nanoparticles for C. gariepinus

From: Effects of ascorbic acid and iron nanoparticles supplements on hyperthermia-induced stress in African catfish, Clarias gariepinus (Burchell, 1822)

 

Treatments

T1 (Control)

T2

T3

T4

T5

Ingredients

Fish meal (72%)

29.00

29.00

29.00

29.00

29.00

Yellow maize (10%)

18.50

18.50

18.50

18.50

18.50

Soybean meal (42%)

29.00

29.00

29.00

29.00

29.00

Rice bran (12%)

12.50

12.50

12.50

12.50

12.50

Soybean oil

6.00

6.00

6.00

6.00

6.00

Vit. / min. premix

2.00

2.00

2.00

2.00

2.00

Cassava Starch

2.00

2.00

2.00

2.00

2.00

Ascorbic acid

1.00

0.80

0.60

0.40

0.00

Iron nanoparticles

0.00

0.20

0.40

0.60

1.00

Parameters (%)

Moisture

19.39 ± 0.05a

10.19 ± 0.17e

10.83 ± 0.10d

18.42 ± 0.09b

11.62 ± 0.09c

Ash

7.16 ± 0.08e

17.83 ± 0.12b

10.51 ± 0.10d

14.31 ± 0.06c

20.60 ± 0.09a

Lipid

3.92 ± 0.04b

3.32 ± 0.10d

3.69 ± 0.08bc

3.67 ± 0.03c

4.76 ± 0.08a

Crude Protein

35.01 ± 0.08a

34.95 ± 0.12b

35.05 ± 0.14a

35.09 ± 0.06a

35.06 ± 0.06a

Crude Fibre

5.02 ± 0.13d

6.70 ± 0.07a

4.59 ± 0.08e

5.42 ± 0.04c

5.85 ± 0.05b

NFE

24.49 ± 0.12c

29.11 ± 0.12b

33.78 ± 0.12a

23.09 ± 0.10d

22.11 ± 0.15e

  1. Nitrogen free extract (NFE) as calculated as 100 – (crude protein + moisture + ether extract + ash + crude fibre)
  2. Figures in each column with the same superscripts are not significantly different (P > 0.05)
  3. Vitamin and mineral premix—Each 1 kg feed contains vitamin A, 1000 mg; vit., D2, 8 mg; vit. E, 7.0 g; vit. K, 0.8 g; vit. B1, 0.49 g, vit. B2, 1.6 g; vit B6 0.6 g; vit. B12 4 mg; Pantothenic acid 49; Nicotinic acid 8 g; Folic acid, 400 mg; Biotin, 20 mg; vit C 40 g; Choline chloride, 200 mg; Copper, 4.0 g; Iodine,0.4 g; Iron, 12 mg; Manganese, 22 g; Zinc 22 g and Selenium 0.04 g – BiotinM, UK
  4. Mean in a given row with the same letter a, b, c, d or e were not significantly different at (P > 0.05)