Carrier RNA is a key factor affecting fully integrated short tandem repeats profiling in challenging forensic samples models

El-Shorbagy, Haidan M.; El-Liethy, Shereen S.; Moussa, Mona K.; Elghor, Akmal A.

doi:10.1186/s41936-022-00286-7

The Journal of Basic and Applied Zoology

Table 3 heterozygous peak ratio (PHR) for each locus from profiles using two extraction methods (EZ1 and organic), “with and without carrier” from two types of fabrics (Black cotton (F1) and denim (F2)), at two storage times (1st and 14th)

From: Carrier RNA is a key factor affecting fully integrated short tandem repeats profiling in challenging forensic samples models

		Carrier	With carrier				Without carrier
		Method	Ez		Manual		Ez		Manual
		Fabric	F1	F2	F1	F2	F1	F2	F1	F2
Ameglonin	Time	1	0.95 ± 0.03b	0.91 ± 0.04ab	0.69 ± 0.13a	0.80 ± 0.14ab	0.90 ± 0.05ab	0.89 ± 0.06ab	0.93 ± 0.02ab	0.94 ± 0.02b
		14	0.91 ± 0.03ab	0.80 ± 0.14ab	0.90 ± 0.07ab	0.91 ± 0.06ab	0.93 ± 0.04ab	0.94 ± 0.02b	0.94 ± 0.02b	0.70 ± 0.09a*
TH01	Time	1	1.00 ± 0.005b	0.97 ± 0.01b	0.65 ± 0.11a	0.97 ± 0.02b	0.92 ± 0.06b	0.90 ± 0.07ab	0.98 ± 0.001b	0.80 ± 0.03ab
		14	0.83 ± 0.08b	0.97 ± 0.03b	0.40 ± 0.03a	0.61 ± 0.14ab	0.86 ± 0.12b	0.80 ± 0.05b	0.74 ± 0.21ab	0.63 ± 0.08ab
D3S1358	Time	1	0.94 ± 0.001b	0.90 ± 0.01ab	0.90 ± 0.04ab	0.93 ± 0.02b	0.94 ± 0.02b	0.93 ± 0.04b	0.58 ± 0.12a	0.79 ± 0.13ab
		14	0.85 ± 0.01a*	0.93 ± 0.02a	0.76 ± 0.17a	0.75 ± 0.04a	0.93 ± 0.001a	0.79 ± 0.13a	0.53 ± 0.02a	0.64 ± 0.05a
VWA	Time	1	0.90 ± 0.09a	0.96 ± 0.01a	0.83 ± 0.08a	0.67 ± 0.18a	0.96 ± 0.03a	0.91 ± 0.07a	0.90 ± 0.06a	0.90 ± 0.06a
		14	0.86 ± 0.03a	0.67 ± 0.18a	0.83 ± 0.08a	0.81 ± 0.08a	0.98 ± 0.01a	0.90 ± 0.06a	0.92 ± 0.04a	0.84 ± 0.07a
D21S11	Time	1	0.88 ± 0.04a	0.88 ± 0.10a	0.79 ± 0.07a	0.84 ± 0.06a	0.88 ± 0.03a	0.90 ± 0.08a	0.90 ± 0.05a	0.91 ± 0.09a
		14	0.79 ± 0.08ab	0.84 ± 0.06ab	0.79 ± 0.01ab	0.69 ± 0.07a	0.97 ± 0.01b*	0.91 ± 0.09ab	0.90 ± 0.03ab	0.69 ± 0.12a
TPOX	Time	1	0.93 ± 0.05a	0.85 ± 0.09a	0.92 ± 0.05a	0.96 ± 0.02a	0.93 ± 0.04a	0.97 ± 0.01a	0.88 ± 0.07a	0.87 ± 0.06a
		14	0.97 ± 0.01b	0.97 ± 0.03b	0.78 ± 0.14a	0.87 ± 0.07ab	0.98 ± 0.01b	0.86 ± 0.06ab	0.89 ± 0.04ab	0.85 ± 0.07ab
D7S820	Time	1	0.85 ± 0.05ab	0.90 ± 0.04b	0.69 ± 0.05a	0.90 ± 0.07b	0.94 ± 0.03b	0.96 ± 0.02b	0.88 ± 0.06b	0.97 ± 0.03b
		14	0.96 ± 0.01b	0.90 ± 0.07b	0.60 ± 0.14a	0.92 ± 0.06b	0.83 ± 0.08ab	0.97 ± 0.03b	0.94 ± 0.03b	0.86 ± 0.02b
D19S433	Time	1	0.88 ± 0.06b	0.92 ± 0.03b	0.91 ± 0.03b	0.39 ± 0.02a	0.82 ± 0.12b	0.91 ± 0.02b	0.87 ± 0.05b	0.95 ± 0.03b
		14	0.81 ± 0.08bc	0.38 ± 0.02a*	0.90 ± 0.07bc	0.66 ± 0.10b	0.85 ± 0.13bc	0.95 ± 0.03bc	0.96 ± 0.02c	0.90 ± 0.03bc
D5S818	Time	1	0.97 ± 0.02^a	0.88 ± 0.05^a	0.74 ± 0.17^a	0.84 ± 0.09^a	0.94 ± 0.03^a	0.96 ± 0.02^a	0.75 ± 0.09^a	0.90 ± 0.07^a
		14	0.94 ± 0.01^a	0.84 ± 0.09^a	0.80 ± 0.07^a	0.77 ± 0.10^a	0.92 ± 0.03^a	0.90 ± 0.07^a	0.76 ± 0.10^a	0.78 ± 0.09^a
D2S1338	Time	1	0.99 ± 0.01^d	0.98 ± 0.01^d	0.81 ± 0.01^bc	0.79 ± 0.06^b	0.96 ± 0.02^d	0.97 ± 0.02^d	0.45 ± 0.04^a	0.91 ± 0.04^cd
		14	0.98 ± 0.01^b	0.79 ± 0.06^a	0.90 ± 0.07^b	0.96 ± 0.02^b	0.99 ± 0.01^b	0.91 ± 0.04^b	0.96 ± 0.02^b*	0.76 ± 0.12^a
D16S539	Time	1	0.99 ± 0.01^b	0.91 ± 0.03^b	0.46 ± 0.03^a	0.47 ± 0.09^a	0.85 ± 0.08^b	0.94 ± 0.01^b	0.60 ± 0.08^a	0.94 ± 0.03^b
		14	0.94 ± 0.05^d	0.47 ± 0.09^ab*	0.31 ± 0.01^a*	0.60 ± 0.03^bc	0.91 ± 0.04^d	0.95 ± 0.03^d	0.76 ± 0.03^c	0.83 ± 0.08^d
CSF1PO	Time	1	0.97 ± 0.02^a	0.97 ± 0.01^a	0.92 ± 0.04^a	0.94 ± 0.04^a	0.99 ± 0.01^a	0.92 ± 0.03^a	0.96 ± 0.02^a	0.97 ± 0.02^a
		14	0.95 ± 0.05^c	0.77 ± 0.18^bc	0.53 ± 0.06^ab*	0.81 ± 0.08^bc	0.91 ± 0.07^c	0.97 ± 0.02^c	0.50 ± 0.04^a*	0.86 ± 0.09^c
D13S317	Time	1	0.96 ± 0.04^b	0.99 ± 0.01^b	0.83 ± 0.08^b	0.90 ± 0.09^b	0.93 ± 0.04^b	0.94 ± 0.01^b	0.83 ± 0.10^b	0.31 ± 0.06^a
		14	0.92 ± 0.04^c	0.90 ± 0.08^c	0.74 ± 0.08^bc	0.60 ± 0.02^b*	0.89 ± 0.05^c	0.32 ± 0.06^a*	0.84 ± 0.02^c	0.39 ± 0.02^a
FGA	Time	1	0.93 ± 0.03^a	0.96 ± 0.02^a	0.80 ± 0.004^a	0.80 ± 0.09^a	0.89 ± 0.04^a	0.77 ± 0.15^a	0.76 ± 0.08^a	0.92 ± 0.04^a
		14	0.97 ± 0.01^b	0.80 ± 0.09^b	0.88 ± 0.09^b	0.94 ± 0.05^b	0.92 ± 0.04^b	0.92 ± 0.04^b	0.86 ± 0.04^b	0.57 ± 0.08^a*
D18S51	Time	1	0.92 ± 0.03^b	0.95 ± 0.03^b	0.95 ± 0.04^b	0.88 ± 0.05^b	0.87 ± 0.05^b	0.87 ± 0.02^b	0.61 ± 0.17^a	0.77 ± 0.11^ab
		14	0.96 ± 0.02^a	0.88 ± 0.05^a	0.94 ± 0.06^a	0.66 ± 0.08^a	0.91 ± 0.04^a	0.77 ± 0.11^a	0.70 ± 0.30^a	0.73 ± 0.10^a
D8S1179	Time	1	0.98 ± 0.01^b	0.93 ± 0.04^b	0.73 ± 0.13^a	0.91 ± 0.04^ab	0.93 ± 0.04^b	0.90 ± 0.05^ab	0.82 ± 0.10^ab	0.98 ± 0.01^b
		14	0.96 ± 0.01^b	0.91 ± 0.04^b	0.94 ± 0.05^b	0.66 ± 0.08^a*	0.97 ± 0.01^b	0.98 ± 0.01^b	0.98 ± 0.02^b	0.91 ± 0.07^b

In the same row, means marked with different superscript letters are significantly different (P < 0.05), whereas those marked with the same ones are insignificantly different (P > 0.05). *: significant difference (P < 0.05), as compared to the value at the first day

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