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The Journal of Basic and Applied Zoology
Fig. 2 | The Journal of Basic and Applied Zoology

Fig. 2

From: Ameliorating effect of blueberry consumption on energy drink-induced testicular damage in rats: histological and immunohistochemical study

Fig. 2

Sections from rat testis immunostained for caspase-3 (apoptotic marker) and photographed at × 100 bar = 200 µm and × 200 bars = 100 µm. Group 1 (Control): with negative immunostaining for caspase-3 in seminiferous tubules (ST) except for mild reaction in interstitial tissue (white arrows). Group 2 (Low dose Code Red): showing desquamated germ cells within ST lumina (thin black arrows) and increased immunostaining for caspase-3 in interstitial cells (thick black arrow). Group 3 (High dose Code Red): showing marked degenerative changes in most ST (Black arrows) and a marked increase in immunostaining for caspase-3 in the degenerated germ cells (black arrows) as well as the interstitial cells (thick black arrows). Group 4 (Low dose Code Red + Blueberry): showing a marked decrease in caspase-3 immunostaining in the interstitial cells (arrows) as well as germ cells (white arrows). Group 5 (High dose Code Red + Blueberry) showed decreased caspase- 3 immunoexpression, although some seminiferous tubules still showed deformity or germ cell desquamation (white arrows)

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