Table 1 The primer’s nucleotide sequences of the rat colon cancer-related genes and housekeeping gene
Gene | Rat primer sequence (5′–3′) | Size of PCR product | Gene accession number |
|---|---|---|---|
β-actin | F: 5′-AGGATGCGTCCACCAAGAAG-3′ R: 5′-TGTCCAGCCCATGATGGTTC-3′ | 249 bp | NM_001101.3 |
COX-2 | F: 5′- TGGTGCCGGGTCTGATGATG-3' R: 5′- GCAATGCGGTTCTGATACTG -3′ | 252 bp | NC_005112.4 |
APC | F: 5′- CAGACTCCAAAAGGGACTGC-3′ R: 5′- TGAATACAAGGGGCGTCTCC -3' | 297 bp | NC_005117.4 |