Hydro-climatic consequences on zooplankton diversity and abundance in perennial Vavuniya reservoir and seasonal Thandikulam reservoir in Vavuniya, Sri Lanka

Abstract

Background

Zooplankton exhibit strong responses for minute changes in their habitat. Therefore, this study intended to investigate the impacts of hydro-climatic factors and related water quality variables on zooplankton abundance and diversity in the perennial Vavuniya reservoir (VR) and seasonal Thandikulam reservoir (TR), Vavuniya, Sri Lanka.

Results

Zooplankton diversity and abundance showed some peculiarities between seasonal and perennial reservoirs under varying hydro-climatic factors and water quality parameters during wet and dry seasons (July 2018–February 2019). There were 14 Rotifera, 4 Cladocera and 5 Copepod species in VR, and in TR, 12 Rotifera, 2 Cladocera and 5 Copepod species were identified. Although these reservoirs are located at proximity (2.48 km), the responses via zooplankton community structure toward environmental factors have been shown uniqueness to some extent. Significant negative correlation was observed for increasing ${\text{NO}}_{{3}}^{ - }$ (p = 0.044) and ${\text{PO}}_{{4}}^{{{3} - }}$ (p = 0.010) concentrations with zooplankton diversity in perennial VR; however, only ${\text{PO}}_{{4}}^{{{3} - }}$ concentration showed a significantly (p = 0.042) negative correlation in seasonal TR. Interestingly, total zooplankton abundance showed significant negative correlations with ${\text{NO}}_{{3}}^{ - }$ (p = 0.013) and ${\text{PO}}_{{4}}^{3 - }$ (p = 0.038) in perennial VR; however, insignificant negative correlation was observed in seasonal TR. Copepods make up the majority of total zooplankton abundance in both reservoirs and were significantly affected by increased atmospheric temperature. Cladocera abundance in VR was highly sensitive (p = 0.032; r = − 0.856) to changes in water level. Increasing water level significantly favors the total zooplankton abundance in the seasonal reservoir but not in perennial.

Conclusions

Sensitivity of zooplankton community structure varies between seasonal and perennial reservoirs in different magnitudes. This is due to the increasing temperatures, increasing phosphate levels and decreasing water levels with the combination of other factors resulting in specific responses of zooplankton diversity and abundance. Therefore, it would not be surprised to use zooplankton community structure to sense the vibrant responses of different freshwater habitats.

Background

Sri Lanka is a tropical country, highly susceptible to climate change. During 1961–1990, country’s atmospheric temperature increased by about 0.02% and mean annual rainfall decreased by around 7% compared to previous 30 years (Eriyagama et al., 2010). Moreover, these climate changes have ability to alter the natural ecosystems associated with water cycle (Climate Change Secretariat; Ministry of Mahaweli Development & Environment, 2016). Zooplankton are tiny aquatic animal component that are not capable to swim against the water current, act as bridge for aquatic food web and often used as environmental indicators (Jeppesen et al., 2011). Due to their short lifespan, zooplankton community structure can be changed rapidly, and it is identifiable within a short time period with environmental changes (Jeppesen et al., 2011; Tian et al., 2017; Zannatul et al., 2009). Zooplankton community depict various preferences for trophic state and the water quality and are recognized to vary predictably with wetland quality and show a rapid respond to surrounding environmental changes (Riato et al., 2014). Recent limnological studies have been more focused on the climate change consequences and zooplankton responses for those changes (Adrian et al., 2009; Vadadi-Fülöp et al., 2012). Hydro-climatic factors were changing with the climate system and climatic variability greatly affects the water resources (Jun & Bantin, 2017). Perennial reservoirs retain water throughout the year, while seasonal reservoirs tend to dry up in latter dry season annually (Impact of Climate Change on culture-based fisheries in seasonal reservoirs & Sri Lanka, 2012). As a seasonal reservoir, Thandikulam reservoir exhibited noticeable water-level changes with the season during this study. Being a perennial, Vavuniya reservoir water-level fluctuations are not vigorous. The study was aimed to detect the hydro-climatic variables, viz. monthly rainfall RF (mm), water level WL (m) and atmospheric temperature AT (°C) as well the water quality parameters, viz. dissolved oxygen level DO (ppm), nitrate level ${\text{NO}}_{3}^{-}$ (ppm), phosphate level ${\text{PO}}_{4}^{3-}$ (ppm), and water temperature WT (°C) on zooplankton community structure (diversity and abundance) in both reservoirs.

Methods

Thandikulam (8° 46′ N, 80° 29′ E) and Vavuniya reservoir (8° 45′ N, 80° 30′ E) are low land dry zone reservoirs, located in Vavuniya district, Northern Province, Sri Lanka (Fig. 1).

Fig. 1

Study sites A₁ Vavuniya reservoir during dry season. A₂ Vavuniya reservoir during wet season. B₁ Thandikulam reservoir during dry season B₂ Thandikulam reservoir during wet season

Four sampling locations of each reservoir were selected randomly and were demarcated by using geographical positioning system (GPS). In order to cover both dry and wet seasons, the sampling was done from July 2018 to February 2019 fortnightly from just below the water surface (10–15 cm). Zooplankton samples were collected in duplicates by filtering 65 litters of water through 80-µm meshed zooplankton net, and the collected samples were immediately preserved with 5% of formalin. Qualitative analysis was done by using standard guides (Mekong River Commission, 2015), and Shannon–Wiener diversity index (H′) (Shannon & Weiner, 1949) was used to find the diversity (Eq. 1).

$$H\prime = - \mathop \sum \limits_{i = 1}^{s} p_{i} \ln p_{i}$$

(1)

where H′ = Shannon–Weiner diversity index, P_i = Fraction of the entire population made up of species I, S = Numbers of species encountered, ∑ = Sum of species 1 to species S.

Quantitative analysis was performed according to Eaton et al. (2005); under compound microscope (Labomed C × L) in low power by using a Sedgwick Rafter cell (Eq. 2).

$$n = \frac{{a \times C \times 1000 \times 10^{6} }}{N \times L}$$

(2)

where n = Total number of plankton in 1 ${\text{m}}^{3}$ (individuals/${\text{m}}^{3}$), a = Total number of plankton in 1 ml, C = Volume of concentrate expressed in ml (here it is 10 × ${10}^{ - 3}$), 1000 = Number of grids in the counting chamber, N = Number of grids employed, L = Volume of water filtered expressed in L (here it is 65 L).

As a result of changing hydro-climatic variables, four major physiochemical parameters were measured during the study, viz. DO, WT, ${\text{NO}}_{{3}}^{ - }$, ${\text{PO}}_{{4}}^{3 - }$. Water temperature was measured from just below the water surface (10–15 cm) by using mercury coiled thermometer. Winkler’s method was used to measure the DO level in water according to US Environmental Protection Agency (1983); ${\text{NO}}_{{3}}^{ - }$ and ${\text{PO}}_{{4}}^{3 - }$ (nutrient) concentration of water was measured by using phenol disulfonic acid method and ascorbic acid-molybdate method, respectively (U.S. Environmental Protection Agency, 1983).

The Pearson correlation matrix (r) was parallelly done for both reservoirs in order to identify the correlations between zooplankton community structures (abundance and diversity) and water quality and hydro-climatic variables by using SPSS 13.0 software.

Results

a) Influence of hydro-climatic and water quality variables on zooplankton species diversity

Total of 23 genera, with 14 Rotifer species, 4 Cladocera species and 5 Copepod, were identified in Vavuniya reservoir (VR), and total of 19 genera with 12 Rotifer species, 2 Cladocera species and 5 Copepod species were observed in Thandikulam reservoir (TR).

Perennial VR showed specific Rotifer species such as Brachionus angularis, B. dimidatus, Keratella cochlearis, Filinia longiseta, Lecane closterocera; Cladocerans such as Ceriodaphnia reticulata, Leptodora kindtii and Copepods such as Tropocyclops sp., while seasonal TR showed unique rotifer species such as B. budapestinensis, Testudinella patina and Lecane bulla.

However, rotifers such as B. angularis, B. calyciflorous, B. caudatus, B. falcatus, B. quadridentatus, B. urceolaris, Keratella tropica, K. valga, Asplanchna sp., Lecane leontina; Cladocera species such as Ceriodaphnia laticaudata, Moina sp.; and Copepoda such as Mesocyclops leukartii, Thermocyclops sp., Calanus sp. were common for both reservoirs (Table 1).

Table 1 Zooplankton species composition in Vavuniya and Thandikulam reservoir during July 2018–February 2019

Appendix 01

Water quality parameters, viz. nitrate concentration and phosphate concentrations, showed a significant negative influence on zooplankton diversity compared to other water quality parameters and hydro-climatic variables (Fig. 2 b1, b2).

Fig. 2

Zooplankton diversity fluctuation in Vavuniya reservoir (left) and Thandikulam reservoir (right) during July 2018–February 2019. a₁ and a₂ ${\text{NO}}_{{3}}^{ - }$ level. b₁ and b₂ ${\text{PO}}_{{4}}^{3 - }$ level

The ${\text{NO}}_{3}^{-}$ concentration was ranged between 0.225 ppm (February 2019) and 1.003 ppm (August 2018) in VR, whereas 0.001 ppm (December 2018) to 2.609 ppm (August 2018) in TR. ${\text{PO}}_{{4}}^{3 - }$ concentration was fluctuated between 0.002 ppm (July 2018) and 0.189 ppm (August 2018) in VR. It was ranged between 0.002 ppm (December 2018) and 0.286 ppm (August 2018) in TR. Zooplankton diversity showed a significant (p = 0.044) negative correlation with ${\text{NO}}_{3}^{-}$ (r = − 0.820) and ${\text{PO}}_{{4}}^{3 - }$ (p = 0.010; r = − 0.936) in VR, whilst, in TR, there was a significant (p = 0.042) negative correlation (r = − 0.805) between diversity with ${\text{PO}}_{4}^{3-}$ but not with ${\text{NO}}_{3}^{-}$ (Table 2).

Table 2 Observed correlation values between environmental parameters and zooplankton diversity index (H')

Further, AT was ranged between 25 °C (February 2019) and 30.1 °C (July 2018) during the study period. Consequently, water temperature in VR was fluctuated between 24 °C (February 2019) and 27 °C (July 2018), and it was recorded between 25 °C (December 2018; January 2019; February 2019) and 26 °C (July 2018; August 2018) in seasonal TR.

A negative correlation was recorded between zooplankton diversity with increasing atmospheric temperature (Fig. 3a1, a2) and RF (Fig. 3b1, b2) in both reservoirs. Perennial reservoir VR showed a positive relationship between zooplankton diversity with increasing WT (Fig. 3a1). However, negative correlation (Fig. 3 a2) was observed in seasonal TR.

Fig. 3

Zooplankton diversity fluctuation in Vavuniya reservoir (left) and Thandikulam reservoir (right) during July 2018–February 2019. a₁ and a₂ Atmospheric and Water temperature. b₁ and b₂ Rainfall

Interestingly, a sudden drop was recorded in DO level in August 2018 and the peak level was recorded in February 2019 in both reservoirs. Numerically it falls between 0.41 ppm and 9.68 ppm in perennial VR, while, in the seasonal TR, the DO level was ranged between 0.41 ppm and 10.09 ppm which was a preferable range for aquatic organisms.

Being a seasonal reservoir, TR showed noticeable fluctuation in WL with the season. 0.3 m depth was recorded in August 2019 (dry season), whereas 2.59 m maximum WL was recorded in January and February 2019 (wet season), while WL in perennial VR was recorded between 2.22 m (July 2018) to 3.71 m (December 2018). Moreover, a positive correlation was detected between zooplankton diversity with increasing WL (Fig. 4 a1, a2) and DO (Fig. 4 b1, b2) in both reservoirs.

Fig. 4

Zooplankton diversity fluctuation in Vavuniya reservoir (left) and Thandikulam reservoir (right) during July 2018–February 2019. a₁ and a₂ Water level. b₁ and b₂ Dissolved oxygen

b) Influence of hydro-climatic and water quality variables on zooplankton abundance

The total zooplankton abundance was ranged between 2462 individuals/${\text{m}}^{3}$ (August 2018) and 9538 individuals/${\text{m}}^{3}$ (February 2019) in VR, while it was ranged between 2461 individuals/${\text{m}}^{3}$ (July and August 2018)—10,153 individuals/${\text{m}}^{3}$ (January 2019) in TR (Table 3). Greater Copepoda abundance was recorded followed by Rotifers and Cladocera.

Table 3 Zooplankton abundance variation (Individual/${\text{m}}^{3}$) during July 2018–February 2019 in Vavuniya and Thandikulam reservoirs

Zooplankton abundance was negatively correlated with AT, WL, ${\text{NO}}_{{3}}^{ - }$ and ${\text{PO}}_{{4}}^{3 - }$ concentrations in VR, while it was positively correlated with DO. However, being a seasonal reservoir, TR exhibits a positive correlation between WL and zooplankton abundance. And there was an inverse relationship between Copepoda zooplankton abundance in TR with temperatures (AT and WT) (Table 4).

Table 4 Correlation coefficient values for zooplankton abundance with environmental parameters

Indeed, atmospheric temperature was one of the major hydro-climatic factors. In Vavuniya reservoir, there was a significant (p = 0.012) negative (r = − 0.926) correlation of total zooplankton abundance with AT, especially, Copepoda abundance depicted a significant (p = 0.046) negative correlation (r = − 0.816) with AT. Interestingly, a significant (p = 0.017) negative correlation (r = − 0.905) was recorded between AT and Copepoda abundance in TR as well (Fig. 5 a1, a2).

Fig. 5

Zooplankton abundance fluctuation with atmospheric temperature and water temperature during July 2018–February 2019. a₁ Vavuniya reservoir. a₂ Thandikulam reservoir

Moreover, a significant (p = 0.001) positive correlation (r = 0.984) was observed in VR zooplankton abundance with increasing DO (Fig. 6 a1, a2).

Fig. 6

Zooplankton abundance fluctuation with dissolved oxygen during July 2018–February 2019. a1 Vavuniya reservoir. a2 Thandikulam reservoir

When it comes to nutrient loading and zooplankton abundance, interestingly perennial VR showed remarkable significant (p = 0.013) negative correlation (r = − 0.922) between zooplankton abundance and ${\text{NO}}_{3}^{-}$ (Fig. 7 a1).

Fig. 7

Zooplankton abundance fluctuation with NO3- level during July 2018–February 2019. a1 Vavuniya reservoir. a2 Thandikulam reservoir

Simultaneously, there was a significant (p = 0.038) negative correlation (r = − 0.839) with ${\text{PO}}_{{4}}^{{3 - }}$ as well (Fig. 8 a1). As noted earlier, this may due to the algal bloom in both reservoirs.

Fig. 8

Zooplankton abundance fluctuation with PO³⁻4 level during July 2018–February 2019. a1 Vavuniya reservoir. a2 Thandikulam reservoir

A significant (p = 0.046) positive (r = 0.817) relationship between WL and total zooplankton abundance in TR was observed in this study. Nevertheless, Cladocera abundance in VR was highly sensitive (p = 0.032; r = − 0.856) to changes in WL (Fig. 9 a1, a2).

Fig. 9

Zooplankton abundance fluctuation with water level during July 2018–February 2019. a1 Vavuniya reservoir. a2 Thandikulam reservoir

A positive correlation was detected in total zooplankton abundance with RF in VR while negative correlation in TR (Fig. 10 a1, a2).

Fig. 10

Zooplankton abundance fluctuation with rainfall during July 2018–February 2019. a1 Vavuniya reservoir. a2 Thandikulam reservoir

Discussion

Limnological assessments are trending in the current world since the lakes and reservoirs are acting as the biological signals for the environmental changes (Williamson et al., 2009).

Interestingly, the study recognized ${\text{PO}}_{{4}}^{3 - }$ as a determining factor for zooplankton diversity in both perennial and seasonal reservoirs than ${\text{NO}}_{3}^{-}$ concentration. Phosphate concentration is a limiting factor on zooplankton diversity in freshwater bodies (Jeppesen et al., 2011). Anthropogenic activities in VR such as industrial activities, especially rice mill effluents, may attribute to the increased nutrient content in the reservoir. According to Aeriyanie et al. (2021), a high abundance of aquatic plants may also lead to nutrient loading in the reservoirs, especially the phosphate content. Therefore, it can be suggested that major reason of nutrient loading in TR could be due the decomposition of its high abundance of submerged aquatic plants. Furthermore, Adrian et al. (2009) state that increased water temperature also can lead to phosphorous accumulation in lakes. Sangakkara and Wijeyaratne (2015) revealed that the dry season in Sri Lanka experienced a high nutrient (${\text{NO}}_{{3}}^{ - }$ and ${\text{PO}}_{{4}}^{3 - }$) loading in reservoirs due to the high evaporation, which may lead to algal bloom, which have the ability to block the filter feeding apparatus of zooplankton, especially in Cladocera species. Ultimately it may induce eutrophication in the reservoirs. And eutrophication negatively influences on the zooplankton diversity in the lakes (Adrian et al., 2009).

Noticeably, zooplankton diversity was declined with increasing ${\text{PO}}_{{4}}^{3 - }$ concentration in both reservoirs (Fig. 2 b1, b2). Bockwoldt et al. (2017) provided a deeper insight to relate the influence of ${\text{PO}}_{{4}}^{3 - }$ concentration on zooplankton diversity in freshwater bodies. According to his records, cyanobacteria density shows an inverse relationship with zooplankton as well as with phytoplankton diversity. Additionally, increased ${\text{PO}}_{{4}}^{3 - }$ concentrations are a stimulus for cyanobacterial blooms. Since cyanobacteria are toxic and less palatable, zooplankton community may undergo food scarcity; which may ultimately reduce their growth, abundance and diversity (Bockwoldt et al., 2017).

Furthermore, Zooplankton diversity variation was observed with RF, WL, AT, DO and WT in each reservoir. Dissolved oxygen also was a crucial factor for zooplankton community structure. According to the studies done by Banerjee et al. (2019); the critical minimum value for the DO in a reservoir is 4.5 ppm. The present study noticed that the DO in August 2018 was remarkably low (0.41 ppm) in both reservoirs, which could be the reason for high zooplankton diversity during the peak DO level periods in both reservoirs. Generally, tropical lakes depict negative correlation between DO and WT, which may ultimately decline the lake biodiversity including zooplankton diversity (Parmesan et al., 2022).

The low abundance of Cladocera and a greater abundance of Copepods, especially nauplii, were a clue for greater predation pressure (El-Bassat, 2008). A greater abundance of copepod nauplii during the wet season could be due to their parthenogenic reproduction strategy. Because of their minute body size, they can avoid the predators (Waya et al., 2017). The same phenomena may exist in TR as well. Although there were optimal conditions for zooplankton community, the total zooplankton abundance was decreased during the February 2019.

Temperature plays a major role in variations of zooplankton community structure. Karpowicz et al. (2020) revealed that, as a result of increasing AT, zooplankton cellular destructions can be occurred due to the exposure of upper surface water column to UV radiation. Larger Copepoda species are highly affected by this phenomenon and will lead to high mortality rates. Therefore, the Copepoda abundance may getting reduced further in both types of reservoirs with increasing AT. Current study revealed a significant positive correlation between AT and WT (r = 0.986; p = 0.001) in TR. Hence, it can suggest that the increasing AT leads to increased WT in TR and it was directly influenced on Copepoda abundance in this seasonal TR to record a significant (p = 0.015) negative correlation (r = − 0.912) with WT.

Though the critical minimum level for DO in a lake is about 4.5 ppm, Crustaceans, viz. Copepoda, can tolerate DO between 1 and 2 ppm with lethal DO 0.3–1.0 ppm (Karpowicz et al., 2020). However, the least value obtained for the DO in both reservoirs was 0.41 ppm, which is not lethal to Copepoda community. Thus, this may attribute with general morphological adaptations of Copepoda such as long and strong appendages to avoid predators to dominant even in low DO concentrations. Supportively, the increasing trend in zooplankton abundance with increasing DO also reported by Manickam et al. (2018) at Tamil Nadu, India.

Nevertheless, during dry season especially in August (2018), dissolved oxygen depletion can be considered as a risk and may lethal to survival of other Rotifers and Caldocerans, which could be a reason for vanishing of Rotifers such as Brachionus angularis, Brachionus budapestinensis, Keratella valga, Keratella cochlearis, Testudinella patina, Lecane bulla, Lecane closterocera, Lecane leontina and Cladocera such as Ceriodaphnia reticulata in both reservoirs.

Additionally, utilization of oxygen in the waterbody for respiration and organic matter decomposition ultimately can cause the decline in DO in the reservoirs (Manickam et al., 2018). Similarly, our present study also reflected as a negative correlation of DO with WT in both reservoirs (r =  − 0.619 in VR; r = − 0.730 in TR).

Increasing ${\text{PO}}_{{4}}^{3 - }$ concentration leads to decline in zooplankton abundance in perennial VR. The studies done by Sangakkara and Wijeyaratne (2015); Haberman and Haldna (2014), for shallow lakes in Estonia and for Sri Lankan low country intermediate zone reservoirs also showed the similar results.

As per the USEPA (2000) standards, both reservoirs have a threat to become eutrophic water bodies in near future since they exceed the standard phosphate concentrations (> 0.024 ppm). Moreover, eutrophication is a phenomenon that can deplete the oxygen in a reservoir (Karpowicz et al., 2020). This can be observed in the current research results since nutrient concentrations (${\text{NO}}_{{3}}^{ - }$ and ${\text{PO}}_{{4}}^{3 - }$) have negative correlation (r = − 0.574, r = − 0.446) with DO in VR and (r = − 0.509, r = − 0.504) in TR, respectively.

Moreover, Sharma and Saini (2016) revealed that the presence of Filinia longiseta is the indicator of industrial effluent impact, and this species also observed in VR that indicates the risk of industrial effluent on VR. Additionally, Vavuniya reservoir is vulnerable to eutrophication that was reflected by the presences of Keratella cochlearis, which is an indicator of eutrophic freshwater bodies.

In addition, nitrate concentration was inversely associated with Copepoda and Rotifera abundance. And ${\text{PO}}_{{4}}^{3 - }$ concentration gives a negative effect on Cladocera community (Jeppesen et al., 2011). This might be the main reason that total zooplankton abundance also shows an inverse relationship with ${\text{NO}}_{{3}}^{ - }$ concentration in our study.

Water level, a crucial hydro-climatic factor, exhibited a noticeable variation between perennial Vavuniya reservoir and seasonal Thandikulam reservoir. Wang et al. (2020) revealed that both Cladocera diversity and abundance are significantly decreasing with increasing water levels. However, increasing WL during December 2018 in VR and January 2019 in TR can reduce the aquatic macrophyte and littoral vegetation, which are very important factors for the growth of Cladocera community. This may lead to loss of DO in freshwater ecosystem.

Supportively, the study also revealed that there was a significantly (p = 0.001) negative correlation (r = − 0.986) of WL with AT. Therefore, it can be suggested that increasing WT through increasing AT led to vaporization of water may attribute to lowering the WL only in the seasonal TR.

Conclusions

This study revealed that zooplankton community structure is highly depended on environmental factors, viz. ${\text{NO}}_{{3}}^{ - }$, ${\text{PO}}_{{4}}^{3 - } ,$ DO, atmospheric temperature and water level in both seasonal Thandikulam and perennial Vavuniya reservoir. Although these reservoirs are located at proximity, sensitivity and responses are seeming to be varied considerably between seasonal and perennial reservoirs.

Indeed, zooplankton community structure is highly sensitive to the nutrient loading in both seasonal and perennial reservoirs. Noticeably, zooplankton diversity and abundance in perennial VR are dependent on ${\text{NO}}_{3}^{-}$ concentration. Further, based on the nutrient loading observations, both reservoirs have a risk to become as a eutrophic water body in near future, which should be taken to consideration. As Filinia longiseta is ample evidence for industrial effluent in VR therefore, rice mill effluent should be monitored and controlled in ordered to conserve this reservoir. In concern of TR, the excess floating vegetation cover created by Eichhornia sp. should be removed to avoid autochthonous carbon and nutrient release into this water body due to the decaying process.

Seasonal reservoirs respond as reduction in zooplankton abundance with respect to decline in water level due to increment in WT and high demand for agricultural activities, which were directly correlated with increasing AT. Therefore, continuous monitoring of zooplankton communities under extreme drought events is necessary under prevailing climate change.

Since the statistical analysis results are aligned with the field observations, the current research is a motivation to use zooplankton community as a bio-sensor to assess environmental alterations in freshwater bodies in local and global context. The use of zooplankton community structure variations to assess the health of existing reservoirs in a cost-effective way is highly recommended.